High-performance detection of Mycobacterium bovis in milk using digital LAMP.
High-performance detection of Mycobacterium bovis in milk using digital LAMP.
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DOI：
10.1016/j.foodchem.2020.126945
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发表时间：
2020-05
期刊：
影响因子：
8.8
通讯作者：
Yi Tao;Juanli Yun;Jian Wang;Peng Xu;Caiming Li;Hongtao Liu;Ying Lan;Jian-zhang Pan;Wenbin Du
中科院分区：
文献类型：
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作者：
Yi Tao;Juanli Yun;Jian Wang;Peng Xu;Caiming Li;Hongtao Liu;Ying Lan;Jian-zhang Pan;Wenbin Du
本文介绍了一种检测牛分枝杆菌（M. bovis）的高效分子检测方法。bovis）。牛瘟是一种持久性病原体，可引起人畜共患结核病，并可感染动物和人类。M.牛乳样品是有效控制和预防人兽共患病的关键，但缺乏有效和敏感的方法。在这里，我们开发了一个方便和低成本的系统为M。牛奶中牛免疫缺陷病毒的检测，结合自动化DNA提取和dLAMP界面乳化技术。与实时PCR相比，dLAMP为directM提供了更高的准确性和灵敏度。牛奶中的牛vis检测，在2小时内提供14 CFU/mL的检测限。dLAMP系统可以成为复杂样品中病原体检测的有力平台，为食品安全检测、流行病学研究和临床诊断提供更可靠的指导。
This paper described a high-performance molecular test for the detection ofMycobacterium bovis(M. bovis) based on digital loop-mediated isothermal amplification (dLAMP).M. bovisis a persistent pathogen that causes zoonotic tuberculosis and can infect both animals and human beings. The detection ofM. bovisin milk samples is critical for effective control and prevention of zoonotic diseases but there lacks effective and sensitive methods. Here, we developed a convenient and low-cost system forM. bovisdetection in milk, which incorporated automated DNA extraction and dLAMP by interfacial emulsification technique. Versus real-time PCR, dLAMP provides higher accuracy and sensitivity for directM. bovisdetection in milk, offering a limit of detection of 14 CFU/mL within 2 h. The dLAMP system can become a powerful platform for the detection of pathogens in complex samples and provide more reliable guidance for food safety testing, epidemiological research and clinical diagnosis.