Comparison of glycolysis and oxidative phosphorylation as energy sources for mammalian sperm motility, using the combination of fluorescence imaging, laser tweezers, and real-time automated tracking and trapping.
Comparison of glycolysis and oxidative phosphorylation as energy sources for mammalian sperm motility, using the combination of fluorescence imaging, laser tweezers, and real-time automated tracking and trapping.
复制标题
DOI：
10.1002/jcp.21549
复制
发表时间：
2008-12
影响因子：
5.6
通讯作者：
Berns, Michael W.
中科院分区：
文献类型：
--
作者：
Nascimento, Jaclyn M.;Shi, Linda Z.;Tam, James;Chandsawangbhuwana, Charlie;Durrant, Barbara;Botvinick, Elliot L.;Berns, Michael W.
激光镊子、荧光成像和实时自动跟踪捕获（RATTS）的组合可以同时测量精子游泳速度和游泳力以及线粒体膜电位（MMP）。该方法用于研究ATP的两种来源在精子运动中的作用：氧化磷酸化，其发生在位于精子中段的线粒体中，以及糖酵解，其发生在沿着精子尾部（鞭毛）的长度。在狗和人精子中研究了（a）游泳速度与MMP和（B）游泳力与MMP之间的关系。本文研究了葡萄糖、氧化磷酸化抑制剂和糖酵解抑制剂对人精子活力的影响。结果表明，氧化磷酸化确实为人类精子运动提供了一些ATP，但不足以维持高运动力。糖酵解途径被证明是人类精子活力的主要能量来源。
The combination of laser tweezers, fluorescent imaging, and real-time automated tracking and trapping (RATTS) can measure sperm swimming speed and swimming force simultaneously with mitochondrial membrane potential (MMP). This approach is used to study the roles of two sources of ATP in sperm motility: oxidative phosphorylation, which occurs in the mitochondria located in the sperm midpiece and glycolysis, which occurs along the length of the sperm tail (flagellum). The relationships between (a) swimming speed and MMP and (b) swimming force and MMP are studied in dog and human sperm. The effects of glucose, oxidative phosphorylation inhibitors and glycolytic inhibitors on human sperm motility are examined. The results indicate that oxidative phosphorylation does contribute some ATP for human sperm motility, but not enough to sustain high motility. The glycolytic pathway is shown to be a primary source of energy for human sperm motility.
登录
查看更多内容
影响因子：
4.9
作者：
Novo, DJ;Perlmutter, NG;Shapiro, HM
通讯作者：
Shapiro, HM
影响因子：
6.1
作者：
Marchetti, C;Obert, G;Marchetti, P
通讯作者：
Marchetti, P
DOI：
10.1073/pnas.0407708101
发表时间：
2004-11-23
影响因子：
11.1
作者：
Miki, K;Qu, WD;O'Brien, DA
通讯作者：
O'Brien, DA
影响因子：
3.9
作者：
Nascimento, Jaclyn M.;Shi, Linda Z.;Berns, Michael W.
通讯作者：
Berns, Michael W.
影响因子：
13.3
作者：
Ford, WCL
通讯作者：
Ford, WCL