Sub-ppm level high energy resolution fluorescence detected X-ray absorption spectroscopy of selenium in articular cartilage
Sub-ppm level high energy resolution fluorescence detected X-ray absorption spectroscopy of selenium in articular cartilage
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DOI：
10.1039/c9an00207c
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发表时间：
2019-06-07
期刊：
影响因子：
4.2
通讯作者：
Bohic, S.
中科院分区：
文献类型：
--
作者：
Bissardon, C.;Proux, O.;Bohic, S.
通过X射线吸收光谱法在各种材料中对高度稀释的元素进行形态分析是极具挑战性的，特别是在生物基质中，如关节软骨。在这里，我们表明，使用高能量分辨率荧光检测X射线吸收光谱（HERFD-XAS）技术耦合到一个阵列的晶体分析仪，硒形态下降到400 ppb（μ g kg（-1））关节软骨内可以证明。这是通过X射线吸收光谱法对高度稀释的元素进行形态分析的重大进展，为研究硒和其他元素在生物样品中的代谢作用开辟了新的可能性。
The speciation of highly-diluted elements by X-ray absorption spectroscopy in a diverse range of materials is extremely challenging, especially in biological matrices such as articular cartilage. Here we show that using a high energy resolution fluorescence detected X-ray absorption spectroscopy (HERFD-XAS) technique coupled to an array of crystal analyzers, selenium speciation down to 400 ppb (mu g kg(-1)) within articular cartilage can be demonstrated. This is a major advance in the speciation of highly-diluted elements through X-ray absorption spectroscopy and opens new possibilities to study the metabolic role of selenium and other elements in biological samples.