Degradation of 4-aminobenzenesulfonate by a two-species bacterial coculture
Degradation of 4-aminobenzenesulfonate by a two-species bacterial coculture
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DOI：
10.1007/bf00058181
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发表时间：
1996-06
期刊：
影响因子：
3.6
通讯作者：
Esther Dangmann;A. Stolz;A. Kuhm;A. Hammer;Burkhard J. Feigel;N. Noisommit-Rizzi;M. Rizzi;M. Reuss;H. Knackmuss
中科院分区：
文献类型：
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作者：
Esther Dangmann;A. Stolz;A. Kuhm;A. Hammer;Burkhard J. Feigel;N. Noisommit-Rizzi;M. Rizzi;M. Reuss;H. Knackmuss
研究了4-氨基苯磺酸盐(磺酸盐)降解混合细菌培养物中的相互作用。该共培养体系由嗜氢噬菌体S1和放射型农杆菌S2组成。在该共培养条件下，只有菌株S1脱氨基生成邻苯二酚-4-磺酸盐，该产物不会在培养基中积累，但可作为菌株S2的生长底物。在以对氨基苯磺酸为唯一碳源、能量源、氮源和硫源的分批培养过程中，两株菌的相对细胞数(菌落形成单位)基本恒定。没有一个菌株的细胞数量比第二个菌株的细胞数量高三倍以上。将两株菌的相对菌落数(相对菌落形成单位数S1：S22200：1~1：500)接种于含对苯磺酸盐的矿物质培养基中。在所有的情况下，都发现了生长，两个菌株的比例接近于大约3：1的值(菌株S1：菌株S2)。与共培养相比，菌株S1不能在含有4-氨基苯磺酸盐或其他任何简单有机化合物的无菌培养中生长。菌株S2的无菌培养上清液能使菌株S1与4-氨基苯磺酸共生。在添加4-氨基苯甲酸酯、生物素和维生素B12的组合后，也发现了同样的促生长作用。菌株S1在4-氨基苯磺酸盐+3种维生素的基础上生长，与矿物质混合培养的生长速度基本相同。当菌株S1的静息细胞在含有磺酸盐的纯矿物培养基中培养时，在培养基中有高达30%的氧化对苯二酚累积为邻苯二酚-4-磺酸盐。相反，当菌株S1在有维生素存在的情况下与4ABS一起培养时，只有少量的儿茶酚-4-磺酸盐积累。
The mutualistic interactions in a 4-aminobenzenesulfonate (sulfanilate) degrading mixed bacterial culture were studied. This coculture consisted ofHydrogenophaga palleroniistrain S1 andAgrobacterium radiobacterstrain S2. In this coculture only strain S1 desaminated sulfanilate to catechol-4-sulfonate, which did not accumulate in the medium but served as growth substrate for strain S2. During growth in batch culture with sulfanilate as sole source of carbon, energy, nitrogen and sulfur, the relative cell numbers (colony forming units) of both strains were almost constant. None of the strains reached a cell number which was more than threefold higher than the cell number of the second strain. A mineral medium with sulfanilate was inoculated with different relative cell numbers of both strains (relative number of colony forming units S1:S2 2200:1 to 1:500). In all cases, growth was found and the proportion of both strains moved towards an about equal value of about 3:1 (strain S1:strain S2). In contrast to the coculture, strain S1 did not grow in a mineral medium in axenic culture with 4-aminobenzenesulfonate or any other simple organic compound tested. A sterile culture supernatant from strain S2 enabled strain S1 to grow with 4-aminobenzenesulfonate. The same growth promoting effect was found after the addition of a combination of 4-aminobenzoate, biotin and vitamin B12. Strain S1 grew with 4-aminobenzenesulfonate plus the three vitamins with about the same growth rate as the mixed culture in a mineral medium. When (resting) cells of strain S1 were incubated in a pure mineral medium with sulfanilate, up to 30% of the oxidized sulfanilate accumulated as catechol-4-sulfonate in the culture medium. In contrast, only minor amounts of catechol-4-sulfonate accumulated when strain S1 was grown with 4ABS in the presence of the vitamins.