Airbrushed nanofibrous membranes to control stem cell infiltration in 3D ‐printed scaffolds
Airbrushed nanofibrous membranes to control stem cell infiltration in 3D ‐printed scaffolds
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DOI：
10.1002/aic.17475
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发表时间：
2021-10
期刊：
影响因子：
3.7
通讯作者：
Chya‐Yan Liaw;Shawn Huynh;Christina Gedeon;Shen Ji;Caroline D’souza;A. Abaci;M. Guvendiren
中科院分区：
文献类型：
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作者：
Chya‐Yan Liaw;Shawn Huynh;Christina Gedeon;Shen Ji;Caroline D’souza;A. Abaci;M. Guvendiren
基于挤出的聚合物生物材料3D打印已成为制造复杂组织工程结构的一种很有前途的方法。然而，较大的孔洞和特征尺寸导致细胞种植效率低，限制了细胞在支架内的空间分布。我们开发了由3D打印层和喷雾纤维膜组成的混合支架。调整喷雾时间以制备低密度(L)、中密度(M)和高密度(H)膜，以有效控制干细胞的渗透。当从支架的顶部或底部种植两个不同的干细胞群体时，由LLL膜组成的支架显示出细胞随深度的逐渐混合，而LHL膜导致两个不同的细胞区域被HL膜分开。我们的结果表明，结合在3D打印层中的纤维膜能够在混合支架中实现用户定义和空间控制的细胞成分。
Extrusion‐based 3D printing of polymeric biomaterials has emerged as a promising approach for the fabrication of complex tissue engineering constructs. However, the large pore and feature size lead to low cell seeding efficiency and limited control of spatial distribution of cells within the scaffolds. We developed hybrid scaffolds that are composed of 3D‐printed layers and airbrushed fibrous membranes. Airbrushing time was adjusted to fabricate low (L), medium (M), and high (H) density membranes to effectively control stem cell infiltration. When two distinct populations of stem cells were seeded from top or bottom of the scaffolds, scaffolds composed ofLLLmembranes showed gradual mixing of the cells with depth, whereasLHLmembranes led to two distinct regions of cells separated by theHmembrane. Our results demonstrate that fibrous membranes incorporated within 3D‐printed layers enable user‐defined and spatially controlled cell compositions within hybrid scaffolds.