Regeneration potential and survival of transplanted undifferentiated adipose tissue-derived stem cells in peripheral nerve conduits
Regeneration potential and survival of transplanted undifferentiated adipose tissue-derived stem cells in peripheral nerve conduits
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DOI：
10.1016/j.bjps.2010.08.013
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发表时间：
2010-12-01
影响因子：
2.7
通讯作者：
Kingham, P. J.
中科院分区：
文献类型：
--
作者：
Erba, P.;Mantovani, C.;Kingham, P. J.
脂肪组织来源的干细胞（ADSCs）已显示出治疗神经损伤的潜力。大多数以前的努力旨在通过使用神经分化方案刺激再生，但未分化的ADSC增强轴突生长的潜力以及它们原位转分化的能力研究得很差。在这项研究中，使用大鼠坐骨神经模型，我们表明，脂肪干细胞，移植在人工神经导管，刺激轴突生长从近端神经残端，并引起更大的雪旺细胞（SC）增殖/入侵在远端残端。为了跟踪移植细胞的命运，我们使用绿色荧光蛋白（GFP）标记和聚合酶链反应（PCR）检测供体雄性细胞中的性别决定区Y（SRY）基因。两种方法均表明移植后14天缺乏显著量的活细胞。这些结果表明，移植的ADSC的任何再生作用更可能是由释放的生长因子的初始增强和/或对内源性SC活性的间接作用介导的。未来的研究需要解决细胞在组织工程神经导管中的长期存活，以提高ADSC的神经再生潜力。(C)2010年英国整形、重建和美容外科医生协会。由爱思唯尔有限公司出版。保留所有权利。
Adipose tissue-derived stem cells (ADSCs) have shown potential for the treatment of nerve injuries. Most previous efforts have aimed at stimulating regeneration by using neural-differentiation protocols, but the potential of undifferentiated ADSCs to enhance axonal growth as well as their ability to transdifferentiate in situ have been poorly investigated. In this study, using a rat sciatic nerve model we show that ADSCs, transplanted in an artificial nerve conduit, stimulate axonal outgrowth from the proximal nerve stump and evoke greater Schwann cell (SC) proliferation/intrusion in the distal stump. To track the fate of the transplanted cells, we used green fluorescent protein (GFP)-labelling and polymerase chain reaction (PCR) for the detection of the sex determining region Y (SRY) gene in the donor male cells. Both methods indicated a lack of significant quantities of viable cells 14 days after transplantation. These results suggest that any regenerative effect of transplanted ADSCs is more likely to be mediated by an initial boost of released growth factors and/or by an indirect effect on endogenous SCs activity. Future studies need to address long-term cell survival in tissue-engineered nerve conduits to improve the neuroregenerative potential of ADSCs. (C) 2010 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.