Analyzing the human response to COVID-19 with arrayed imaging reflectometry
Analyzing the human response to COVID-19 with arrayed imaging reflectometry
复制标题
使用阵列成像反射计分析人类对 COVID-19 的反应
DOI：
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
B. Miller
中科院分区：
文献类型：
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作者：
B. Miller
事实证明，在 COVID-19 时代，对抗体检测工具的迫切需求尤为迫切。基于阵列的工具是评估更广泛的抗原特异性反应模式的方法，以及在对其他病毒已有免疫力的情况下提供有关 SARS-CoV-2 免疫力的信息的方法。为此，本演讲将描述用于快速量化 SARS-CoV-2 和其他上呼吸道病原体抗体的无标记微阵列的开发。该方法建立在阵列成像反射计（AIR）的基础上，这是一种无标记检测技术，基于硅芯片表面抗反射涂层的产生和结合诱导的降解。将讨论使用一组人类样本（恢复期的 COVID-19 患者和阴性对照）的阵列性能以及在筛选单克隆抗体中的应用。
The urgent need for antibody detection tools has proven particularly acute in the COVID-19 era. Array-based tools are desirable as methods for assessing broader patterns of antigen-specific responses, as well as for providing information on SARS-CoV-2 immunity in the context of pre-existing immunity to other viruses. To that end, this talk will describe the development of a label-free microarray for rapidly quantifying antibodies to SARS-CoV-2 and other upper respiratory pathogens. The approach is built on Arrayed Imaging Reflectometry (AIR), a label-free detection technique based on the creation and binding-induced degradation of an antireflective coating on the surface of a silicon chip. Performance of the array using a cohort of human samples (convalescent COVID-19 patients and negative controls) along with applications in screening monoclonal antibodies will be discussed.