Enhanced transepithelial antigen transport in intestine of allergic mice is mediated by IgE/CD23 and regulated by interleukin-4
Enhanced transepithelial antigen transport in intestine of allergic mice is mediated by IgE/CD23 and regulated by interleukin-4
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DOI：
10.1053/gast.2001.26470
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发表时间：
2001-08-01
期刊：
影响因子：
29.4
通讯作者：
Perdue, MH
中科院分区：
文献类型：
--
作者：
Yu, LCH;Yang, PC;Perdue, MH
背景和目标：我们先前描述了一种用于增强抗原跨旋转转运的系统，在该系统中，特定抗原的量及其运输速率在敏化大鼠的肠道中显着增加，与对照组相比。这项研究研究了介导小鼠中介导的抗原摄取的必需成分，用于白介素（IL）-4或CD23。方法：小鼠对辣根过氧化物酶（HRP）积极或被动地敏感。来自对照或敏化小鼠的空肠段安装在Ussing Charbers中，并从腔侧挑战了HRP。对组织进行电子显微镜处理，并分析显微照片的抗原摄取（含HRP的内体的位置和面积）。免疫组织化学和反转录聚合酶链反应用于检测上皮CD23表达。结果：积极敏化的IL-4（+/+），而不是IL-4（ - / - ）小鼠，在肠上皮细胞上显示出增加的跨维抗原转运和CD23的表达。免疫血清转移后，被动敏化的IL-4+/+和IL-4 - / - 小鼠显示出抗原转运升高，但如果血清被免疫球蛋白（Ig）E或IL-4耗尽，则不会。 IL-4添加到培养的IEC-4细胞上上调CD23信使RNA的表达。抗CD23抑制了增强的抗原摄取，并且在敏化的CD23（ - / - ）小鼠中不存在。结论：我们的研究表明，IL-4在敏化小鼠肠中调节IgE/CD23介导的增强的旋转抗原转运。
Background & Aims: We previously described a system for enhanced transepithelial transport of antigen in which both the amount of specific antigen and its rate of transport were dramatically increased in intestine of sensitized rats compared with controls. This study investigated the essential components mediating antigen uptake in mice genetically deficient for interleukin (IL)-4 or CD23. Methods: Mice were actively or passively sensitized to horseradish peroxidase (HRP). Jejunal segments from control or sensitized mice were mounted in Ussing chambers and challenged with HRP from the luminal side. Tissues were processed for electron microscopy, and photomicrographs were analyzed for antigen uptake (location and area of HRP-containing endosomes). Immunohistochemistry and reverse-transcription polymerase chain reaction were used to detect epithelial CD23 expression. Results: Actively sensitized IL-4(+/+), but not IL-4(-/-) mice, displayed increased transepithelial antigen transport and CD23 expression on enterocytes. Passively sensitized IL-4+/+ and IL-4-/- mice displayed elevated antigen transport after transfer of Immune serum but not if the serum was depleted of immunoglobulin (Ig) E or IL-4. IL-4 added to cultured IEC-4 cells up-regulated expression of CD23 messenger RNA. The augmented antigen uptake was inhibited by anti-CD23 and was absent in sensitized CD23(-/-) mice. Conclusions: Our studies indicate that IL-4 regulates IgE/CD23-mediated enhanced transepithelial antigen transport in sensitized mouse intestine.