Selenium Deficiency Influences the mRNA Expression of Selenoproteins and Cytokines in Chicken Erythrocytes
Selenium Deficiency Influences the mRNA Expression of Selenoproteins and Cytokines in Chicken Erythrocytes
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缺硒影响鸡红细胞硒蛋白和细胞因子mRNA表达
DOI：
10.1007/s12011-015-0536-8
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发表时间：
2016-06-01
影响因子：
3.9
通讯作者：
Xu, Shiwen
中科院分区：
文献类型：
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作者：
Luan, Yilin;Zhao, Jinxin;Xu, Shiwen
硒（Se）缺乏诱导鸡溶血，但这种效应的分子机制尚不清楚。硒主要通过硒蛋白的活性发挥其功能，硒蛋白含有独特的氨基酸硒代半胱氨酸（Sec）。本研究旨在探讨硒缺乏对24种硒蛋白和10种细胞因子表达的影响。将180只鸡随机分为2组，每组90只。在整个实验期间，允许鸡自由采食饲料和水。分别饲喂低硒日粮（0.008 mg Se/kg;产自中国黑龙江省缺硒地区）或补硒日粮（以亚硒酸钠计）（0.2 mg/kg）35 d。35日龄时，检测各组5只鸡红细胞中24种硒蛋白和10种细胞因子的mRNA水平，并进行相关性分析。结果显示，缺硒组24种硒蛋白和7种细胞因子（IL-2、IL-4、IL-8、IL-10、IL-12β、TGF-β4和IFN-γ）的表达均降低（P< 0.05），而3种细胞因子（IL-1γ、IL-6和IL-7）的表达升高（P< 0.05）。两组鸡红细胞中谷胱甘肽过氧化物酶（GPX）、硫氧还蛋白1（Txnrd 1）、硒蛋白P1（SELP）和硒蛋白合成酶（SPS 2）的表达量均高于其它硒蛋白（P< 0.05）。这些数据表明，GPXs，Txnrd 1，SELP和SPS 2可能发挥更重要的作用比其他硒蛋白。促炎细胞因子（IL-1γ、IL-6和IL-7）的升高提示硒缺乏对鸡免疫系统有损伤作用。相关性分析表明，尽管24种硒蛋白和7种细胞因子的表达量降低，3种细胞因子的表达量升高，但它们的表达量与硒饮食密切相关。结果表明，硒缺乏影响了鸡红细胞24种硒蛋白和10种细胞因子的表达，揭示了硒与鸡免疫系统的关系。这项研究提供了有关硒缺乏诱导溶血的机制的信息。
Selenium (Se) deficiency induces hemolysis in chickens, but the molecular mechanism for this effect remains unclear. Se primarily elicits its function through the activity of selenoproteins, which contain the unique amino acid selenocysteine (Sec). In this study, we aimed to investigate the effect of Se deficiency on the expression of 24 selenoproteins and 10 cytokines. One hundred eighty chickens were randomly divided into 2 groups (90 chickens per group). During the entire experimental period, chickens were allowed ad libitum consumption of feed and water. The chickens were fed either a Se-deficient diet (0.008 mg Se/kg; produced in the Se-deficient area of Heilongjiang, China) or a Se-supplemented diet (as sodium selenite) at 0.2 mg/kg for 35 days. At the 35th day, the messenger RNA (mRNA) levels of 24 selenoproteins and 10 cytokines were examined in erythrocytes of 5 chickens per group, and the correlation was analyzed. The results showed that the expression of 24 selenoproteins and 7 cytokines (IL-2, IL-4, IL-8, IL-10, IL-12β, TGF-β4, and IFN-γ) decreased (P< 0.05), and the expression of 3 cytokines (IL-1γ, IL-6 and IL-7) was higher in the Se-deficient group. In both groups, glutathione peroxidase (GPX), thioredoxin 1 (Txnrd1), selenoprotein P1 (SELP), and selenoprotein synthetase (SPS2) were highly expressed compared to the other selenoproteins in chicken erythrocytes (P< 0.05). These data suggest that GPXs, Txnrd1, SELP, and SPS2 possibly play a more important role than the other selenoproteins. The increase of pro-inflammatory cytokines (IL-1γ, IL-6, and IL-7) suggested that the immune system of chickens was damaged by the Se deficiency. Correlation analysis suggested that although the expression of 24 selenoproteins and 7 cytokines decreased and that of 3 cytokines increased, there was a close correlation between their expression levels and a Se diet. These results suggested that Se deficiency influenced the expressions of 24 selenoproteins and 10 cytokines in chicken erythrocytes, revealing a relationship between Se and the chicken immune system. This study offers information regarding the mechanism of Se deficiency-induced hemolysis.