The basal level ethylene response is important to the wall and endomembrane structure in the hypocotyl cells of etiolated Arabidopsis seedlings.
The basal level ethylene response is important to the wall and endomembrane structure in the hypocotyl cells of etiolated Arabidopsis seedlings.
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DOI：
10.1111/j.1744-7909.2012.01130.x
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发表时间：
2012-07
影响因子：
11.4
通讯作者：
Chan Xu;X. Gao;X. Sun;C. Wen
中科院分区：
文献类型：
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作者：
Chan Xu;X. Gao;X. Sun;C. Wen
通过观察黄化拟南芥幼苗下胚轴细胞的超微结构，研究了乙烯调节的细胞伸长过程中发生的亚细胞事件。抑制乙烯反应促进了细胞伸长，细胞表现出壁疏松和分离的表型。附近的壁分离的网站经常与皮质粗面内质网（ER）膜的增加，壁旁体的存在下，和环状高尔基体的形成。蛋白质生物合成抑制剂放线菌酮可逆转皮质rER增殖和环状高尔基体表型。当乙烯反应被阻止时，皮质rER膜较长，而随着乙烯反应的升高而缩短。野生型和乙烯不敏感突变体乙烯不敏感2（ein 2）幼苗下胚轴之间的蛋白质组变化表明，不同的蛋白质亚群涉及内膜运输，重塑和壁修饰的差异表达。FM 4 -64染色支持蛋白质组学变化，这表明随着乙烯反应的缓解，内吞活性降低。乙烯的基础水平反应在内膜运输、生物物质运输和内膜组织的维持中起着重要作用。内膜的改变可能与细胞壁的改变有关，但其生物学意义有待进一步研究。
The sub-cellular events that occur during the ethylene-modulated cell elongation were characterized by examining the ultra-structure of etiolated Arabidopsis seedling hypocotyl cells. Preventing the basal level ethylene response facilitated cell elongation, and the cells exhibited wall loosening and separation phenotype. Nearby the wall separation sites were frequently associated with an increase in the cortical rough endoplasmic reticulum (rER) membranes, the presence of paramural bodies, and the circular Golgi formation. The cortical rER proliferation and circular Golgi phenotype were reverted by the protein biosynthesis inhibitor cycloheximide. The cortical rER membranes were longer when the ethylene response was prevented and shortened with elevated ethylene responses. Proteomic changes between wild type and the ethylene-insensitive mutant ethylene insensitive2 (ein2) seedling hypocotyls indicated that distinct subsets of proteins involving endomembrane trafficking, remodeling, and wall modifications were differentially expressed. FM4-64 staining supported the proteomic changes, which indicated reduced endocytosis activity with alleviation of the ethylene response. The basal level ethylene response has an important role in endomembrane trafficking, biological materials transport and maintenance of the endomembrane organization. It is possible that endomembrane alterations may partly associate with the wall modifications, though the biological significance of the alterations should be addressed in future studies.