High resolution micro-XRF maps of iron oxides inside sensory dendrites of putative avian magnetoreceptors
High resolution micro-XRF maps of iron oxides inside sensory dendrites of putative avian magnetoreceptors
复制标题
假定鸟类磁感受器感觉树突内氧化铁的高分辨率微 XRF 图
DOI：
10.1088/1742-6596/186/1/012084
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Janssens
中科院分区：
文献类型：
--
作者：
Falkenberg;Fleissner;Fleissner;Schuchardt;Kuehbacher;Chalmin;Janssens
信鸽[1]和多种鸟类[2]上喙内壁含有铁矿物的感觉树突是鸟类磁场感受器的第一个候选结构。一种新的磁感受概念[3，4]是基于详细的超微结构光学和电子显微镜分析，结合同步辐射显微X射线荧光分析（micro-XRF）和显微X射线吸收近边结构（micro-XANES）。几个行为实验[5，6]和第一个数学模拟[6]证实了我们的鸟类磁感受器模型。树突内的铁矿物被容纳在三个不同的亚细胞区室（子弹，血小板，囊泡）中，可以通过电子显微镜在切片上清楚地分辨和识别[1，3]。在HASYLAB光束线L处获得的Micro-XRF和Micro-XANES数据增加了关于元素分布和Fe形态的信息[3]，但是由于有限的空间分辨率而在整个枝晶上平均。在这里，我们提出了最近进行的微XRF地图与亚微米分辨率（ESRF ID 21），这揭示了第一次从几乎散装样的树突样品材料的亚细胞结构信息。由于厚度为30 μm，因此可以认为树突的微结构未受干扰，并且切片引入的伪影可能会大大减少。
Iron mineral containing sensory dendrites in the inner lining of the upper beak of homing pigeons [1] and various bird species [2] are the first candidate structures for an avian magnetic field receptor. A new concept of magnetoreception [3, 4] is based on detailed ultra-structural optical and electron microscopy analyses in combination with synchrotron radiation microscopic X-ray fluorescence analysis (micro-XRF) and microscopic X-ray absorption near edge structures (micro-XANES). Several behavioral experiments [5, 6] and first mathematical simulations [6] affirm our avian magnetoreceptor model. The iron minerals inside the dendrites are housed in three different subcellular compartments (bullets, platelets, vesicles), which could be clearly resolved and identified by electron microscopy on ultrathin sections [1, 3]. Micro-XRF and micro-XANES data obtained at HASYLAB beamline L added information about the elemental distribution and Fe speciation [3], but are averaged over the complete dendrite due to limited spatial resolution. Here we present recently performed micro-XRF maps with sub-micrometer resolution (ESRF ID21), which reveal for the first time subcellular structural information from almost bulk-like dendrite sample material. Due to the thickness of 30 μm the microarchitecture of the dendrites can be considered as undisturbed and artefacts introduced by sectioning might be widely reduced.
影响因子：
3.4
作者：
I. Solov’yov;W. Greiner
通讯作者：
W. Greiner
影响因子：
1.3
作者：
Fleissner, Gerta;Fleissner, Guenther;Falkenberg, Gerald
通讯作者：
Falkenberg, Gerald