Response of the Nrf2 protection system in human monocytic cells after ionising iIrradiation.
Response of the Nrf2 protection system in human monocytic cells after ionising iIrradiation.
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电离辐射后人单核细胞中 Nrf2 保护系统的反应。
DOI：
10.1093/rpd/ncs201
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发表时间：
2012
影响因子：
1
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Hironori Yoshino;et al.
作为对活性氧（ROS）或亲电体的响应，转录因子核因子-红细胞2（NF-E2）相关因子2（Nrf 2）迅速易位到细胞核中并诱导各种抗氧化基因的表达，如血红素加氧酶-1（HO-1）。低线性能量转移（LET）电离辐射（如X射线）会产生ROS，导致生物损伤。然而，很少有人知道是否在人类单核细胞中的Nrf 2系统被激活低LET电离辐射。因此，在本研究中，研究了人单核细胞THP 1细胞中Nrf 2系统对X射线照射的响应。THP 1细胞暴露于X射线（1-5戈伊）后，用2′，7 ′-二氯二氢荧光素二乙酸酯测定细胞内ROS水平，用免疫荧光染色测定Nrf 2定位，并检测HO-1 mRNA和蛋白表达。虽然ROS产生的辐射剂量依赖性的方式，他们立即消失后照射。在5戈伊X射线照射后6 h观察到Nrf 2易位入核，但在1-2戈伊照射后或未照射对照中未检测到。HO-1的表达显着高于5 Gy照射后24小时的细胞比未照射的对照组。这些结果表明，高剂量照射（5戈伊）激活Nrf 2，并且Nrf 2保护系统可能从照射后24小时起在人单核细胞中发挥作用。
In response to reactive oxygen species (ROS) or electrophiles, the transcription factor nuclear factor-erythroid 2 (NF-E2)-related factor 2 (Nrf2) rapidly translocates into the nucleus and induces the expression of various antioxidant genes, such as heme oxygenase-1 (HO-1). Low linear energy transfer (LET) ionising radiations such as X-rays generate ROS, which cause biological damage. However, little is known about whether the Nrf2 system in human monocytic cells is activated by low LET ionising irradiation. Therefore, in this study, the response of the Nrf2 system to X-irradiation in human monocytic THP1 cells was investigated. Following exposure of THP1 cells to X-rays (1–5 Gy), intracellular ROS levels were measured using 2′,7′-dichlorodihydrofluorescein diacetate, Nrf2 localisation was determined using immunofluorescence staining and HO-1 mRNA and protein expression were examined. Although ROS were generated by irradiation in a dose-dependent manner, they disappeared immediately after irradiation. Nrf2 translocation into the nucleus was observed 6 h after 5 Gy X-irradiation but was not detected following 1–2 Gy irradiation or in non-irradiated controls. HO-1 expression was significantly higher in 5 Gy-irradiated cells after 24 h than in non-irradiated controls. These results indicate that high-dose irradiation (5 Gy) activates Nrf2 and that the Nrf2 protection system may function from 24 h after irradiation in human monocytic cells.