Comparison of Nostocean hormogonium induction and its motility on solid plates between agar and gellan gum at varying gel matrix concentrations
Comparison of Nostocean hormogonium induction and its motility on solid plates between agar and gellan gum at varying gel matrix concentrations
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不同凝胶基质浓度下琼脂和结冷胶在固体板上的发菜激素诱导及其运动性的比较
DOI：
10.1080/09168451.2017.1420464
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Hiroaki Nishizuka and Yasuyuki Hashidoko
中科院分区：
文献类型：
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作者：
Takashi Okubo;Takeshi Tokida;Seishi Ikeda;Zhihua Bao;Kanako Tago;Masahito Hayatsu;Hirofumi Nakamura;Hidemitsu Sakai;Yasuhiro Usui;Kentaro Hayashi;Toshihiro Hasegawa;Kiwamu Minamisawa;Hiroaki Nishizuka and Yasuyuki Hashidoko
为了建立发菜激素诱导的灵敏生物测定法，我们比较了两种凝胶板（琼脂和结冷胶）对漆树酸 C15:1-Δ8 癸酯 (1)（100 nmol/盘）形态分化诱导的有效性。在 BG-110（无氮）培养基 0.6 和 0.8% 琼脂平板上，发菜。从屋久岛苏铁的珊瑚状根中分离得到的菌株 Yaku-1 表现出从丝状聚集体到激素原的明显形态分化，并且诱导的激素原在 24 小时内分散；然而，在琼脂浓度为 1.0% 或更高时，没有观察到类似的激素形成。相反，即使在孵育 12 小时后，激素诱导在结冷胶平板上比琼脂平板上的激素诱导更为明显，浓度范围为 0.6% 至 1.6%，即使在孵育 12 小时后，特别是在 0.8-1.0% 结冷胶平板上活跃。因此，结冷胶平板可以在 12 小时内获得清晰的结果，因此对于激素诱导因子 (HIF) 的初步筛选非常有用。
To establish a sensitive bioassay for Nostocean hormogonium induction, we compared the effectiveness of the morpho-differentiation induction on two gelled plates, agar and gellan gum, for anacardic acid C15:1-Δ8decyl ester (1) (100 nmol/disc). On BG-110(nitrogen-free) medium-based 0.6 and 0.8% agar plates,Nostocsp. strain Yaku-1 isolated from a coralloid root ofCycas revolutain Yakushima Island showed clear morpho-differentiation from filamentous aggregates into hormogonia, and the induced hormogonia dispersed within 24 h; however, similar hormogonium formation was not observed at agar concentrations of 1.0% or higher. Conversely, hormogonium induction was considerably more pronounced on gellan gum plates than those on agar plates through concentrations ranging from 0.6 to 1.6% even after 12 h of incubation, particularly active on the 0.8–1.0% gellan gum plates. Thus, gellan gum plates can achieve clear results within 12 h and are thus highly useful for primary screening for hormogonium-inducing factors (HIFs).