mRNA length-sensing in eukaryotic translation: reconsidering the "closed loop" and its implications for translational control.
mRNA length-sensing in eukaryotic translation: reconsidering the "closed loop" and its implications for translational control.
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DOI：
10.1007/s00294-016-0674-3
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发表时间：
2017-08
期刊：
影响因子：
2.5
通讯作者：
Gilbert WV
中科院分区：
文献类型：
--
作者：
Thompson MK;Gilbert WV
大多数真核生物 mRNA 通过识别 5' m7GpppN 帽而被募集至核糖体。 30 年的遗传和生化证据表明，5'帽相互作用因子和 3'poly(A) 结合蛋白之间的相互作用在使 mRNA 末端紧密靠近并促进 mRNA 的翻译和稳定性方面发挥着重要作用，其形式称为闭环。然而，最近的 RNA-蛋白质相互作用研究结果表明，并非所有 mRNA 都能平等地接触闭环因子。此外，与闭环因子的关联似乎强烈偏向具有短开放阅读框的 mRNA，这与在许多真核生物中观察到的短 mRNA 更高翻译的趋势相呼应。我们最近报道，核糖体信号支架蛋白 RACK1 促进与闭环因子密切相关的短 mRNA 的有效翻译。在这里，我们讨论了这些观察结果对翻译控制的影响，并提出了重新审视真核翻译闭环普遍性的途径。
Most eukaryotic mRNAs are recruited to the ribosome by recognition of a 5’ m7GpppN cap. 30 years of genetic and biochemical evidence point to a role for interaction between the 5’ cap-interacting factors and the 3’ poly(A)-binding protein in bringing the ends of the mRNA into close proximity and promoting both translation and stability of the mRNA, in a form known as the closed loop. However, the results of recent RNA-protein interaction studies suggest that not all mRNAs have equal access to the closed loop factors. Furthermore, association with closed loop factors appears to be strongly biased towards mRNAs with short open reading frames, echoing the trend for higher translation of short mRNAs that has been observed in many eukaryotes. We recently reported that the ribosomal signaling scaffold protein RACK1 promotes the efficient translation of short mRNAs that strongly associate with the closed loop factors. Here, we discuss the implications of these observations with respect to translational control and suggest avenues through which the universality of the closed loop in eukaryotic translation could be revisited.