Alteration of antral and proximal colonic motility induced by chronic psychological stress involves central urocortin 3 and vasopressin in rats
Alteration of antral and proximal colonic motility induced by chronic psychological stress involves central urocortin 3 and vasopressin in rats
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DOI：
10.1152/ajpgi.00390.2011
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发表时间：
2012-08-01
影响因子：
4.5
通讯作者：
Fujimiya, Mineko
中科院分区：
文献类型：
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作者：
Ataka, Koji;Nagaishi, Kanna;Fujimiya, Mineko
[10]张文，张文.慢性心理应激引起大鼠胃窦和近端结肠运动的改变涉及中枢尿皮质素3和加压素。美国生理学杂志胃肠和肝脏生理学303：G519-G528，2012年。首次发表于2012年5月25日; doi：10.1152/ajpgi.00390.2011。由于难以建立合适的动物模型，应激诱导的功能性胃肠病的发病机制尚不清楚。本研究应用交流箱技术诱导大鼠心理应激，并检测其胃肠动力。我们测量了急性和慢性心理应激引起的上、下消化道运动，并检测了下丘脑中各种神经肽的mRNA表达。慢性心理应激可使胃窦的禁食运动中断，近端结肠的运动加速。AVP、催产素和尿皮质素3的mRNA表达增加。脑室内（ICV）注射urocortin 3破坏了空腹胃窦运动，而ICV注射UCN 3抗血清防止慢性心理应激引起的胃窦运动的改变。ICV注射AVP加速结肠运动，而ICV注射SSR 149415，一种选择性AVP V1 b受体拮抗剂，防止慢性心理应激引起的近端结肠运动的改变。催产素及其受体拮抗剂L371257对正常或慢性心理应激模型的结肠运动均无影响。这些结果表明，慢性心理应激引起的通信盒技术可能会破坏空腹运动在胃窦通过尿皮质素3途径和加速近端结肠运动通过AVP V1 b受体在大脑中。
Ataka K, Nagaishi K, Asakawa A, Inui A, Fujimiya M. Alteration of antral and proximal colonic motility induced by chronic psychological stress involves central urocortin 3 and vasopressin in rats. Am J Physiol Gastrointest Liver Physiol 303: G519-G528, 2012. First published May 25, 2012; doi:10.1152/ajpgi.00390.2011.-Because of the difficulties in developing suitable animal models, the pathogenesis of stress-induced functional gastrointestinal disorders is not well known. Here we applied the communication box technique to induce psychological stress in rats and then examined their gastrointestinal motility. We measured upper and lower gastrointestinal motility induced by acute and chronic psychological stress and examined the mRNA expression of various neuropeptides in the hypothalamus. Chronic psychological stress disrupted the fasted motility in the antrum and accelerated motility in the proximal colon. mRNA expression of AVP, oxytocin, and urocortin 3 was increased by chronic psychological stress. Intracerebroventricular (ICV) injection of urocortin 3 disrupted the fasted motility in the antrum, while ICV injection of Ucn3 antiserum prevented alteration in antral motility induced by chronic psychological stress. ICV injection of AVP accelerated colonic motility, while ICV injection of SSR 149415, a selective AVP V1b receptor antagonist, prevented alteration in proximal colonic motility induced by chronic psychological stress. Oxytocin and its receptor antagonist L 371257 had no effect on colonic motility in either the normal or chronic psychological stress model. These results suggest that chronic psychological stress induced by the communication box technique might disrupt fasted motility in the antrum via urocortin 3 pathways and accelerates proximal colonic motility via the AVP V1b receptor in the brain.