Identification of stress-responsive genes in an indica rice (Oryza sativa L.) using ESTs generated from drought-stressed seedlings
Identification of stress-responsive genes in an indica rice (Oryza sativa L.) using ESTs generated from drought-stressed seedlings
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DOI：
10.1093/jxb/erl213
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发表时间：
2007-01-01
影响因子：
6.9
通讯作者：
Reddy, Arjula R.
中科院分区：
文献类型：
--
作者：
Gorantla, Markandeya;Babu, P. R.;Reddy, Arjula R.
干旱对植物生长和发育的影响限制了世界范围内的谷类作物生产。水稻（Oryza sativa）的生产力和产量由于几乎所有农业生态区的经常性干旱而受到严重影响。随着分子和基因组技术的出现，现在的重点是了解干旱胁迫反应的遗传控制机制。为了鉴定水稻中与水分胁迫反应相关的基因，使用了从籼稻品种纳吉纳22的干旱胁迫叶片组织构建的标准化cDNA文库中产生的EST。通过对7794个cDNA序列的分析，共鉴定出5815个水稻EST。其中，334个与公开数据库中的任何水稻EST或全长cDNA没有显着的序列同源性，表明这些转录本在干旱胁迫期间富集。对这5815个EST的分析导致了1677个独特序列的鉴定。为了进一步描述干旱转录组的特征，并确定与干旱胁迫反应相关的候选基因，将水稻数据与从拟南芥、大麦、玉米和水稻的表达谱研究中获得的非生物胁迫诱导序列进行了比较。这项比较分析确定了589个假定的压力响应基因（SRG），这些不同的植物物种共享。此外，将鉴定的叶SRGs与干旱胁迫水稻穗文库的表达谱进行比较，以鉴定共同序列。值得注意的是，125个基因被发现在干旱胁迫下表达在这两个组织。对这125个基因的功能分类表明，它们中的大多数与细胞代谢、信号转导和转录调控有关。
The impacts of drought on plant growth and development limit cereal crop production worldwide. Rice (Oryza sativa) productivity and production is severely affected due to recurrent droughts in almost all agroecological zones. With the advent of molecular and genomic technologies, emphasis is now placed on understanding the mechanisms of genetic control of the drought-stress response. In order to identify genes associated with water-stress response in rice, ESTs generated from a normalized cDNA library, constructed from drought-stressed leaf tissue of an indica cultivar, Nagina 22 were used. Analysis of 7794 cDNA sequences led to the identification of 5815 rice ESTs. Of these, 334 exhibited no significant sequence homology with any rice ESTs or full-length cDNAs in public databases, indicating that these transcripts are enriched during drought stress. Analysis of these 5815 ESTs led to the identification of 1677 unique sequences. To characterize this drought transcriptome further and to identify candidate genes associated with the drought-stress response, the rice data were compared with those for abiotic stress-induced sequences obtained from expression profiling studies in Arabidopsis, barley, maize, and rice. This comparative analysis identified 589 putative stress-responsive genes (SRGs) that are shared by these diverse plant species. Further, the identified leaf SRGs were compared to expression profiles for a drought-stressed rice panicle library to identify common sequences. Significantly, 125 genes were found to be expressed under drought stress in both tissues. The functional classification of these 125 genes showed that a majority of them are associated with cellular metabolism, signal transduction, and transcriptional regulation.