SNAP RECEPTORS IMPLICATED IN VESICLE TARGETING AND FUSION
SNAP RECEPTORS IMPLICATED IN VESICLE TARGETING AND FUSION
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DOI：
10.1038/362318a0
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发表时间：
1993-03-25
期刊：
影响因子：
64.8
通讯作者：
ROTHMAN, JE
中科院分区：
文献类型：
--
作者：
SOLLNER, T;WHITEHART, SW;ROTHMAN, JE
N-乙基马来酰亚胺敏感性融合蛋白（NSF）和可溶性NSF附着蛋白（SNAP）似乎是细胞内膜融合装置的重要组成部分。基于这些蛋白质与其靶标的天然结合的亲和纯化程序用于从牛脑中分离SNAP受体（SNARE）。值得注意的是，分离出的四种主要蛋白质都是与突触相关的蛋白质，一种位于突触囊泡中，另一种位于质膜中，这表明囊泡对接的简单机制。许多SNARE相关蛋白的存在，每一个显然是特定的一种囊泡或靶膜，表明NSF和SNAP可能是一个囊泡融合器的通用组件共同组成和调节融合（包括神经递质释放），其中SNARE可能有助于确保囊泡靶特异性。
The N-ethylmaleimide-sensitive fusion protein (NSF) and the soluble NSF attachment proteins (SNAPs) appear to be essential components of the intracellular membrane fusion apparatus. An affinity purification procedure based on the natural binding of these proteins to their targets was used to isolate SNAP receptors (SNAREs) from bovine brain. Remarkably, the four principal proteins isolated were all proteins associated with the synapse, with one type located in the synaptic vesicle and another in the plasma membrane, suggesting a simple mechanism for vesicle docking. The existence of numerous SNARE-related proteins, each apparently specific for a single kind of vesicle or target membrane, indicates that NSF and SNAPs may be universal components of a vesicle fusion apparatus common to both constitutive and regulated fusion (including neurotransmitter release), in which the SNAREs may help to ensure vesicle-to-target specificity.