Retinoic acid restores normal growth control to a transformed mouse embryo fibroblast cell line.
Retinoic acid restores normal growth control to a transformed mouse embryo fibroblast cell line.
复制标题
视黄酸可恢复转化小鼠胚胎成纤维细胞系的正常生长控制。
DOI：
10.1016/0304-3835(86)90099-6
复制
发表时间：
1986
期刊：
影响因子：
9.7
通讯作者：
Brattain,MG
中科院分区：
文献类型：
--
作者：
Levine,AE;Crandall,CA;Brattain,D;Chakrabarty,S;Brattain,MG
研究了维甲酸对转化的小鼠胚胎成纤维细胞系（AKR-MCA）的作用。用视黄酸处理以剂量依赖性方式恢复了该转化细胞系的非转化表型。视黄酸（RA）处理的AKR-MCA细胞表现出非转化形态，生长速度较慢，并且不具有锚定独立性。与未转化的AKR-2B细胞系相比，在AKR-MCA转化的细胞系中38，000 Da蛋白质被高度磷酸化。RA处理大大降低了AKR-MCA细胞中该蛋白的磷酸化水平。生长停滞的AKR-MCA细胞对营养补充有促有丝分裂反应，但对表皮生长因子（EGF）无反应。用RA处理AKR-MCA细胞恢复了它们对EGF的反应能力，而对营养补充的反应丧失。这种生长控制模式与未转化的AKR-2B细胞的生长控制模式相似。
The effects of retinoic acid on a transformed mouse embryo fibroblast cell line (AKR-MCA) were examined. Treatment with retinoic acid restored a non-transformed phenotype to this transformed cell line in a dose dependent manner. Retinoic acid (RA) treated AKR-MCA cells showed a non-transformed morphology, a slower growth rate, and did not grow with anchorage independence. A 38,000 Da protein was phosphorylated to a high degree in the AKR-MCA transformed cell line compared to the non-transformed AKR-2B cell line. RA treatment greatly reduced the level of phosphorylation of this protein in AKR-MCA cells. Growth arrested AKR-MCA cells showed a mitogenic response to nutrient replenishment, but not to epidermal growth factor (EGF). Treatment of AKR-MCA cells with RA restored their ability to respond to EGF while the response to nutrient replenishment was lost. This pattern of growth control was similar to that of the non-transformed AKR-2B cells.