Phytochemical Engineered Bacterial Outer Membrane Vesicles for Photodynamic Effects Promoted Immunotherapy
Phytochemical Engineered Bacterial Outer Membrane Vesicles for Photodynamic Effects Promoted Immunotherapy
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植物化学工程细菌外膜囊泡的光动力效应促进免疫治疗
DOI：
10.1021/acs.nanolett.2c01280
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Hai-Yan Xie
中科院分区：
文献类型：
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作者：
Wan-Ru Zhuang;Yunfeng Wang;Yao Lei;Liping Zuo;Anqi Jiang;Guanghao Wu;Weidong Nie;Li-Li Huang;Hai-Yan Xie
癌症疫苗正在成为肿瘤免疫治疗的一种有吸引力的方式。然而，其实际应用由于复杂的制造和不令人满意的结果而受到严重阻碍。在此，我们构建了基于细菌外膜囊泡（OMV）的原位癌症疫苗，具有植物化学特征，用于光动力效应促进的免疫治疗。通过简单地将类囊体膜与 OMV 融合，即可制备细菌-植物混合囊泡 (BPN)。全身给药后，BPNs 可以靶向肿瘤组织并刺激免疫细胞的激活，包括树突状细胞 (DC)。类囊体产生的光动力效应导致局部肿瘤的破坏，然后释放由 DC 有效呈递的肿瘤相关抗原，诱导显着的肿瘤特异性 CD8+T 细胞反应。此外，BPNs可以有效改善免疫抑制的肿瘤微环境，进一步增强免疫反应。因此，可以有效地预防肿瘤的发展和转移。这项工作为开发用于高效肿瘤治疗的多功能膜混合系统提供了新的思路。
Cancer vaccines are emerging as an attractive modality for tumor immunotherapy. However, their practical application is seriously impeded by the complex fabrication and unsatisfactory outcomes. Herein, we construct bacterial outer membrane vesicles (OMVs)-based in situ cancer vaccine with phytochemical features for photodynamic effects-promoted immunotherapy. By simply fusing thylakoid membranes with OMVs, bacteria-plant hybrid vesicles (BPNs) are prepared. After systemic administration, BPNs can target tumor tissues and stimulate the activation of immune cells, including dendritic cells (DCs). The photodynamic effects derived from thylakoid lead to the disruption of local tumors and then the release of tumor-associated antigens that are effectively presented by DCs, inducing remarkable tumor-specific CD8+T cell responses. Moreover, BPNs can efficiently ameliorate the immunosuppressive tumor microenvironment and further boost immune responses. Therefore, both tumor development and metastasis can be efficiently prevented. This work provides a novel idea for developing a versatile membrane-based hybrid system for highly efficient tumor treatment.