Induction of secretory pathway components in yeast is associated with increased stability of their mRNA.
Induction of secretory pathway components in yeast is associated with increased stability of their mRNA.
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DOI：
10.1083/jcb.200112008
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发表时间：
2002-03-18
期刊：
影响因子：
--
通讯作者：
Meyer DI
中科院分区：
文献类型：
--
作者：
Hyde M;Block-Alper L;Felix J;Webster P;Meyer DI
某些膜蛋白的过度表达伴随着细胞内膜的显著增殖。膜增殖的最佳表征诱导物之一是180-kD哺乳动物核糖体受体（p180），其在酵母中的表达导致编码在分泌途径中起作用的蛋白质的mRNA水平增加，以及细胞分泌蛋白质的能力的提高。在这项研究中，我们表明，无论是未折叠的蛋白质反应，也没有增加转录占膜增殖或分泌途径mRNA的增加。相反，p180诱导的上调某些分泌途径的转录是由于p180介导的这些mRNA种类的寿命增加，通过测量转录活性和特定的mRNA周转。此外，我们表明，mRNA的寿命一般是通过其靶向内质网膜的过程中大大促进。关于分泌组织的终末分化，从这个模型系统的结果提供了一个单一的蛋白质，p180，如何表达的见解，可能会导致大量的形态和功能的变化。
The overexpression of certain membrane proteins is accompanied by a striking proliferation of intracellular membranes. One of the best characterized inducers of membrane proliferation is the 180-kD mammalian ribosome receptor (p180), whose expression in yeast results in increases in levels of mRNAs encoding proteins that function in the secretory pathway, and an elevation in the cell's ability to secrete proteins. In this study we demonstrate that neither the unfolded protein response nor increased transcription accounts for membrane proliferation or the observed increase in secretory pathway mRNAs. Rather, p180-induced up-regulation of certain secretory pathway transcripts is due to a p180-mediated increase in the longevity of these mRNA species, as determined by measurements of transcriptional activity and specific mRNA turnover. Moreover, we show that the longevity of mRNA in general is substantially promoted through the process of its targeting to the membrane of the endoplasmic reticulum. With respect to the terminal differentiation of secretory tissues, results from this model system provide insights into how the expression of a single protein, p180, could result in substantial morphological and functional changes.
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