Maximum titers of vitellogenin and total hemolymph protein occur during the canalized phase of grasshopper egg production
Maximum titers of vitellogenin and total hemolymph protein occur during the canalized phase of grasshopper egg production
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DOI：
10.1086/324475
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发表时间：
2001-11-01
影响因子：
1.6
通讯作者：
Juliano, SA
中科院分区：
文献类型：
--
作者：
Hatle, JD;Borst, DW;Juliano, SA
许多生物只在敏感阶段表现出发育可塑性，在其他时候不能对环境扰动作出反应。然而，我们知道的生理事件，定义塑料和渠化阶段。在昆虫产卵过程中，卵黄原蛋白（Vg）首先在血淋巴中积累，然后在卵中积累。此外，贮藏蛋白可能是鸡蛋生产的重要资源。因此，我们测试的Vg和TP（总血淋巴蛋白减去Vg）滴度的关系，从灵活的过渡到不灵活的发展过程中的鸡蛋生产的假设。在Lubber蚱蜢中，类似于70%的TP包含在三种蛋白质中，其范围从68至83 kDa。我们保持女性的食物处理，产生定义的塑料和渠化时期，收集血淋巴每近似4天，并确定产卵和最大Vg和TP滴度发生的年龄。Vg（max）滴度，尤其是TPmax滴度都是产蛋数的预测因子。取食对羽化至Vg（max）的时间有显著影响，但对Vg（max）至产卵的时间无显著影响。同样，从羽化到TPmax的时间受饲料的影响显著，而从TPmax到产卵的时间没有。因此，Vg（max）和TPmax是在产蛋的管道化阶段期间发生的生理标志。
Many organisms exhibit developmental plasticity only in sensitive phases and cannot respond to environmental pertubations at other times. However, we know little about the physiological events that define plastic and canalized phases. During egg production in insects, vitellogenin (Vg) accumulates first in the hemolymph and then in the eggs. In addition, storage proteins may be important resources for egg production. Therefore, we tested hypotheses on the relationships of Vg and TP (total hemolymph protein minus Vg) titers to the transition from flexible to inflexible development during egg production. In lubber grasshoppers, similar to 70% of TP is contained in three proteins that range from 68 to 83 kDa. We maintained females on food treatments that produced defined plastic and canalized periods, collected hemolymph every similar to4 d, and determined the ages at which oviposition and the maximum Vg and TP titers occurred. Both Vg(max) titer and especially TPmax titer were predictors of the number of eggs produced. The time from eclosion to Vg(max) was significantly affected by diet, but the time from Vg(max) to oviposition was not. Similarly, the time from eclosion to TPmax was significantly affected by diet, while the time from TPmax to oviposition was not. Hence, Vg(max) and TPmax are physiological landmarks that occur during the canalized phase of egg production.