Transcriptome analysis of porcine immature oocytes and surrounding cumulus cells after vitrification and in vitro maturation
Transcriptome analysis of porcine immature oocytes and surrounding cumulus cells after vitrification and in vitro maturation
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玻璃化和体外成熟后猪未成熟卵母细胞和周围卵丘细胞的转录组分析。
DOI：
10.1016/j.theriogenology.2019.05.019
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发表时间：
2019-08-01
期刊：
影响因子：
2.8
通讯作者：
Wu, Guo-Quan
中科院分区：
文献类型：
--
作者：
Jia, Bao-Yu;Xiang, De-Cai;Wu, Guo-Quan
冷冻保存会损害卵母细胞的质量，这可能与基因表达异常有关。目前，猪卵母细胞玻璃化冷冻后mRNA水平的变化还没有得到很好的描述。本研究旨在利用RNA测序技术分析猪未成熟卵母细胞及其周围卵丘细胞(CCS)玻璃化冷冻和体外成熟(IVM)后的转录组特征。在玻璃化冷冻的卵母细胞中，有19个表达上调的基因和18个表达下调的基因存在差异表达，这些基因没有发现明显的GO浓缩或KEGG途径。此外，来自玻璃化冷冻卵母细胞的CCS有40个基因显著上调，100个基因显著下调。KEGG分析表明，共有7个GO项在分子功能和生物学过程中显著丰富，只有MAPK信号通路达到显著丰富。此外，通过比较qRT-PCR和RNA-Seq的结果，筛选出的差异表达基因具有相似的表达模式。总之，我们的数据提供了关于猪未成熟卵母细胞和玻璃化冷冻和体外成熟后CCS的mRNA转录的详细信息，这为现在关于玻璃化冷冻卵母细胞发育潜力降低的研究提供了新的见解。(C)2019 Elsevier Inc.保留所有权利。
Cryopreservation impairs oocyte quality, which may be associated with abnormal gene expression. Currently, alteration of mRNA levels in vitrified porcine oocytes has not been well characterized. The aim of this study was to analyze transcriptome profiles with RNA sequencing (RNA-seq) in porcine immature oocytes and their surrounding cumulus cells (CCs) after vitrification and in vitro maturation (IVM). There were 19 upregulated and 18 downregulated genes differentially expressed in vitrified oocytes, with no significant GO enrichment or KEGG pathway identified for these genes. In addition, CCs derived from vitrified oocytes had 40 significantly upregulated and 100 significantly downregulated genes. In total, 7 GO terms were significantly enriched in molecular function and biological process, and only MAPK signaling pathway reached significant enrichment based on KEGG analysis. Moreover, selected differentially expressed genes had similar expression patterns through comparison between results from qRT-PCR and RNA-Seq. In conclusion, our data provided detailed information on mRNA transcriptomes in porcine immature oocytes and CCs after vitrification and IVM, which offered now insights regarding reduced developmental potential of the vitrified oocytes. (C) 2019 Elsevier Inc. All rights reserved.