Immunosensors: Electrochemical sensing and other engineering approaches
Immunosensors: Electrochemical sensing and other engineering approaches
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DOI：
10.1016/s0956-5663(97)00031-6
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发表时间：
1998-01-01
影响因子：
12.6
通讯作者：
Wilkins, E
中科院分区：
文献类型：
--
作者：
Ghindilis, AL;Atanasov, P;Wilkins, E
本文综述了替代免疫分析系统的工程方法和最新发展趋势。简要介绍了常规免疫分析的主要原理和局限性。讨论了克服这些限制的免疫传感方法。从增强免疫相互作用过程和各种检测原理的角度讨论了传统免疫分析系统的替代方案。介绍了流动注射技术在免疫传感系统开发中的应用。免疫传感器根据检测原理分为免疫电极(电化学免疫传感器)、压电式免疫传感器或基于免疫相互作用的光学检测的传感器。讨论的重点是电化学免疫传感器。最后，概述了免疫传感器发展中涉及的工程问题，并讨论了实际应用的趋势。(C)1998年爱思唯尔科学有限公司。
This article overviews the engineering approaches and the recent trends in the development of alternative immunoassay systems. A brief description of the main principles and limitations of conventional immunoassay is given. Immunosensing approaches overcoming these limitations are discussed. Alternatives to traditional immunoassay systems are discussed in terms of the enhancement of immunointeraction processes and in terms of the various detection principles. Applications of flow-injection techniques to the development of immunosensing systems are presented. Immunosensors are categorized based on the detection principle employed, as immunoelectrodes (electrochemical immunosensors), piezoelectric immunosensors, or as sensors based on optical detection of the immunointeraction. The discussion focuses on electrochemical immunosensors. In conclusion, the engineering issues involved in immunosensor development are outlined and trends towards practical applications are discussed. (C) 1998 Elsevier Science Limited.