Cochlear dimensions obtained in hemicochleae of four different strains of mice: CBA/CaJ, 129/CD1, 129/SvEv and C57BL/6J
Cochlear dimensions obtained in hemicochleae of four different strains of mice: CBA/CaJ, 129/CD1, 129/SvEv and C57BL/6J
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DOI：
10.1016/s0378-5955(01)00374-4
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发表时间：
2001-12-01
期刊：
影响因子：
2.8
通讯作者：
Richter, CP
中科院分区：
文献类型：
--
作者：
Keiler, S;Richter, CP
因为老鼠和人类基因组的同源性。由于两者的遗传性异常是相似的，小鼠为研究人类遗传性听力障碍提供了一个有价值的动物模型。遗传性听力障碍的表现之一可能是耳蜗元件的结构，例如耳蜗的大体形态。然而，耳蜗的尺寸是决定内耳力学和听力功能的一个因素。因此，当比较不同品系小鼠的听力时，总耳蜗尺寸可能很重要。虽然一些研究已经在亚细胞水平上检查了小鼠内耳结构，但只有少数研究耳蜗的大体形态。此外，可用的稀疏数据是从固定和脱水的组织中获得的。然而，脱水会导致耳蜗盖膜和基底膜透明基质等凝胶状结构严重变形。在这项研究中，hemicocolera技术，它允许新鲜的小鼠耳蜗材料被视为从径向的角度来看，被用来提供一个逐项研究的总耳蜗结构的尺寸在四个小鼠品系（CBA/CaJ，129/SvEv，129/CDI和C57 BL/6 J）。除了CBA/CaJ之外，已知这些菌株具有与年龄相关的听力损失的基因。测量结果表明，四种菌株之间没有重大差异。然而，与以前的数据相比，基底膜的厚度测量值大了10倍。这种差异可能是由于处理材料所用的技术不同造成的。这种半交叉技术消除了以前实验中出现的脱水引起的大部分失真。(C)2001 Elsevier Science B. V.保留所有权利。
Because homologies between mice and human genomes. are well established and hereditary abnormalities are similar in both, mice present a valuable animal model to study hereditary hearing disorders in humans. One of the manifestations of hereditary hearing disorders might be in the structure of cochlear elements, such as the gross morphology of the cochlea. Cochlear dimensions, however, are one factor that determines inner ear mechanics and thus hearing function. Therefore, gross cochlear dimension might be important when different strains of mice are compared regarding their hearing. Although several studies have examined mouse inner ear structures on a sub-cellular level, only few have studied cochlear gross morphology. Moreover, the sparse data available were acquired from fixed and dehydrated tissue. Dehydration, however, prod-aces severe distortion of gel-like cochlear structures such as the tectorial membrane and the basilar membrane hyaline matrix. In this study, the hemicochlea technique, which allows fresh mouse cochlear material to be viewed from a radial perspective, was used to provide an itemized study of the dimensions of gross cochlear structures in four mouse strains (CBA/CaJ, 129/SvEv, 129/CDI and C57BL/6J). Except for the CBA/CaJ, these strains are known to possess genes for age-related hearing loss. The measurements showed no major differences among the four strains. However, when compared with previous data, the thickness measures of the basilar membrane were up to 10 times larger. Such differences are likely to result from the different techniques used to process the material. The hemicochlea technique eliminates much of the distortion caused by dehydration, which was present in previous experiments. (C) 2001 Elsevier Science B.V. All rights reserved.