Bcl-2 and p53 protein expression, apoptosis, and p53 mutation in human epithelial ovarian cancers
Bcl-2 and p53 protein expression, apoptosis, and p53 mutation in human epithelial ovarian cancers
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DOI：
10.1016/s0002-9440(10)64744-x
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发表时间：
2000-02-01
影响因子：
6
通讯作者：
Mok, SC
中科院分区：
文献类型：
--
作者：
Chan, WY;Cheung, KK;Mok, SC
Bcl-2和p53基因产物都与细胞凋亡有关细胞死亡。在本研究中，我们通过免疫组织化学定位研究了正常人卵巢中Bcl-2和p53蛋白表达，p53突变和凋亡的关系，以及不同类型的人卵巢上皮肿瘤。链反应链构象多态性。发现Bcl-2在正常卵巢，良性和边界卵巢肿瘤的表面上皮上强烈表达，但在恶性肿瘤中弱。相反，在检查的54％（25/46）中发现了p53的强蛋白表达。在检查的恶性卵巢肿瘤中发现-2和p53表达。然而，外显子5-11处的p53基因突变并不是p53表达在边缘和恶性肿瘤中的先决条件。凋亡指数反映的凋亡活性在正常的卵巢表面上皮中很低。良性肿瘤，但在边缘和恶性肿瘤中增加，在III级恶性肿瘤中发现的平均凋亡指数最高。统计分析显示凋亡与p53表达之间存在正相关，但是在凋亡与Bcl-2表达之间没有发现相似的相关性。我们的结果还表明，尽管在卵巢癌发生过程中，Bcl-2的表达很重要，但Bcl-2蛋白除了成为人类卵巢上皮癌的凋亡调节剂外，还具有其他作用。
Bcl-2 and p53 gene products have been both linked to cell death by apoptosis. In the present study, we examined the relationship of Bcl-2 and p53 protein expression,p53 mutation and apoptosis in normal human ovaries and different types of human ovarian epithelial tumors by immunohistochemical localization, in situ terminal transferase-mediated dUTP nick end labeling and polymerase chain reaction-single strand conformation polymorphism. It was found that Bcl-2 expressed strongly in the surface epithelium of normal ovaries and benign and borderline ovarian tumors but weakly in the malignant tumors. On the contrary, strong protein expression of p53 was found in 54% (25/46) of the malignant epithelial tumors examined but similar expression of p53 was not observed in borderline and benign tumors and normal ovarian surface epithelium, A significant inverse correlation between Bcl-2 and p53 expression was found in the malignant ovarian tumors examined. p53 gene mutation at exons 5-11 was however not a pre-requisite for p53 expression in both borderline and malignant tumors. Apoptotic activities, as reflected by apoptotic indices, were low in normal ovarian surface epithelium and. benign tumors but were increased in borderline and malignant tumors, with the highest average apoptotic index found in grade III malignant tumors. Statistical analyses showed a positive correlation between apoptosis and p53 expression, but similar correlation was not found between apoptosis and Bcl-2 expression. Our results also indicate that although expression of Bcl-2 is important during ovarian carcinogenesis, the Bcl-2 protein may have other roles to play apart from being a modulator of apoptosis in human ovarian epithelial cancers.