Leishmania donovani lipophosphoglycan disrupts phagosome microdomains in J774 macrophages
Leishmania donovani lipophosphoglycan disrupts phagosome microdomains in J774 macrophages
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DOI：
10.1111/j.1462-5822.2005.00550.x
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发表时间：
2005-09-01
影响因子：
3.4
通讯作者：
Desjardins, M
中科院分区：
文献类型：
--
作者：
Dermine, JF;Goyette, G;Desjardins, M
通过吞噬作用清除病原体并在吞噬溶酶体中杀死病原体是我们与生俱来的抗感染能力的一个关键方面。利什曼原虫通过抑制吞噬体成熟和避免吞噬溶酶体的恶劣环境，进化出在巨噬细胞中生存和复制的方式。我们在这里描述，在此过程中，杜氏利什曼原虫使用一种新的策略，涉及其表面脂磷酸聚糖（LPG），一种毒力因子阻碍许多主机功能，以防止形成或破坏吞噬体膜上的脂质微区。LPG局部作用于膜，并需要其重复的碳水化合物部分来改变微区的组织。靶向和破坏功能病灶，其中蛋白质参与吞噬溶酶体生物发生的关键方面组装，可能会赋予寄生虫的生存优势。
Clearance of pathogens by phagocytosis and their killing in phagolysosomes is a key aspect of our innate ability to fight infectious agents. Leishmania parasites have evolved ways to survive and replicate in macrophages by inhibiting phagosome maturation and avoiding the harsh environment of phagolysosomes. We describe here that during this process Leishmania donovani uses a novel strategy involving its surface lipophosphoglycan (LPG), a virulence factor impeding many host functions, to prevent the formation or disrupt lipid microdomains on the phagosome membrane. LPG acts locally on the membrane and requires its repetitive carbohydrate moieties to alter the organization of microdomains. Targeting and disruption of functional foci, where proteins involved in key aspects of phagolysosome biogenesis assemble, is likely to confer a survival advantage to the parasite.