A new solvent system for the separation of neutral glycosphingolipids.
A new solvent system for the separation of neutral glycosphingolipids.
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用于分离中性糖鞘脂的新溶剂系统。
DOI：
10.4049/jimmunol.133.2.835
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发表时间：
1981
影响因子：
6.5
通讯作者：
Y. Arao
中科院分区：
文献类型：
--
作者：
K. Watanabe;Y. Arao
描述了用于无需衍生化即可分离鞘糖脂的高效液相色谱的溶剂系统和色谱柱。使用填充有多孔硅胶(latbeads)并用异丙醇-己烷-水的混合物洗脱的柱，其中水含量增加且己烷含量减少。具有单糖、十二糖或十糖的鞘糖脂可在 60 分钟内分离，并且分离模式具有高度可重复性。该方法适用于具有血型活性的高度复杂糖脂的制备分离。
A solvent system and a column for high performance liquid chromatography for the separation of glycosphingolipids without derivatization is described. A column pakced with porous silica gel (latrobeads) and eluted with a mixture of isopropanol-hexane-water with increasing water content and decreasing hexane content was used. Glycosphingolipids with mono- to dodeca- or tetrakaidecasaccharides were separated within 60 min and the separation pattern was highly reproducible. The method was applied for preparative separation of highly complex glycolipids with blood group activity.