P-selectin-dependent adhesion of human cancer-cells - requirement for coexpression of a psgl-1-like core protein and the glycosylation process for sialosyl-le(x) or sialosyl-le(a).
P-selectin-dependent adhesion of human cancer-cells - requirement for coexpression of a psgl-1-like core protein and the glycosylation process for sialosyl-le(x) or sialosyl-le(a).
复制标题
人类癌细胞的 P-选择素依赖性粘附 - 需要 psgl-1 样核心蛋白的共表达以及 sialosyl-le(x) 或 sialosyl-le(a) 的糖基化过程。
DOI：
10.3892/ijo.6.4.773
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发表时间：
1995
影响因子：
5.2
通讯作者：
S. Hakomori
中科院分区：
文献类型：
--
作者：
K. Handa;T. White;K. Ito;H. Fang;Ss Wang;S. Hakomori
我们研究了唾液酸-Le（x）（SLe（x））和唾液酸-Le（a）（SLe（a））的细胞表面表达及其与E-选择素和P-选择素依赖性细胞粘附的相关性，采用12种来自实体瘤的人癌细胞系和2种髓性白血病细胞系HL 60和U937。在所有检测的细胞系中，E-选择素依赖性粘附与SLe（x）和SLe（a）表达程度之间存在明显的相关性。实体瘤细胞系与P-选择素无显著结合，但白血病细胞系HL 60和U937与P-选择素强烈结合。将编码P-选择素糖蛋白配体-1（PSGL-1）的cDNA克隆转染到结肠癌HRT 18和肺癌PC 3细胞中，所述细胞表达SLe（x）和SLe（a），但通常不结合P-选择素，尽管它们结合E-选择素。所得转染子与P-选择素强烈结合，与E-选择素同样良好结合。从合并的人结肠癌组织中提取的粗粘蛋白级分与E-选择素结合，但不与P-选择素结合。我们的结论是，肿瘤细胞粘附P-选择素是高度依赖于一个特定的核心蛋白的表达，适当地组装一个特定的碳水化合物，目前P-选择素。相比之下，E-选择素通过N-连接、O-连接或脂质连接的结构混杂地结合细胞表面呈递的各种类型的SLe（x）和SLe（a）表位。
We studied the cell surface expression of sialosyl-Le(x) (SLe(x)) and sialosyl-Le(a) (SLe(a)) and its correlation with E-selectin- and P-selectin-dependent cell adhesion, employing 12 human cancer cell lines derived from solid tumors and 2 myelogenic leukemic cell lines, HL60 and U937. Among all the cell lines tested, there was a clear correlation between E-selectin-dependent adhesion and degree of SLe(x) and SLe(a) expression. None of the cell lines derived from solid tumors bound significantly to P-selectin, but leukemic cell lines HL60 and U937 bound strongly to P-selectin. The cDNA clone encoding P-selectin glycoprotein ligand-1 (PSGL-1) was transfected into colonic cancer HRT18 and lung cancer PC3 cells, which express SLe(x) and SLe(a) but normally do not bind to P-selectin, although they do bind to E-selectin. The resulting transfectants bound strongly to P-selectin and equally well to E-selectin. A crude mucin fraction extracted from pooled human colonic cancer tissue bound to E-selectin but not to P-selectin. We conclude that tumor cell adhesion to P-selectin is highly dependent on expression of a specific core protein which appropriately assembles a specific carbohydrate to present to P-selectin. In contrast, E-selectin binds promiscuously to various types of SLe(x) and SLe(a) epitopes presented at the cell surface through N-linked, O-linked, or lipid-linked structures.