Differentiation of mixtures of co-product blend with barley grain based on Fourier transform infrared attenuated total reflection molecular spectroscopy: Carbohydrate molecular spectral profiles and nutritive characteristics in dairy cattle
Differentiation of mixtures of co-product blend with barley grain based on Fourier transform infrared attenuated total reflection molecular spectroscopy: Carbohydrate molecular spectral profiles and nutritive characteristics in dairy cattle
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DOI：
10.3168/jds.2012-5450
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发表时间：
2012-11-01
影响因子：
3.5
通讯作者：
Yu, Peiqiang
中科院分区：
文献类型：
--
作者：
Zhang, Xuewei;Yu, Peiqiang
本研究的目的是：(1)基于傅里叶变换红外衰减全反射分子光谱聚类分析，区分混合副产物（生物乙醇发酵前小麦：玉米的70%:30%混合物）与大麦谷物加工生物乙醇的混合物；(2)阐明混合副产物对奶牛无壳大麦碳水化合物（CHO）的分子结构、光谱特征和营养特性的影响。(3)定量分析奶牛CHO分子结构光谱与混合物CHO营养特性的关系。CHO分子结构光谱分析的参数包括木质素、结构CHO （STCHO）、纤维素化合物和总CHO的分子光谱峰面积和高度强度。CHO营养特性的评估参数包括CHO营养特征、CHO亚组分、能量值和瘤胃可发酵有机质供应。分别按100:0 （B100）、75:25、50:50、25:75和0:100 （BD100）的比例混合去壳大麦籽粒和副产物混合物。结果表明，当以不同比例添加副产物混合料时，瘤胃可发酵有机物的预测干物质供应量（DM; BD100）从632 g/kg显著降低至518 g/kg。瘤胃有效可降解干物质和有效可降解淀粉含量也分别从672和482 g/kg DM （B100）显著降低至475和43 g/kg DM （B100）。通过傅里叶变换红外衰减全反射光谱，我们发现木质素、STCHO、纤维素化合物、总CHO光谱峰面积、峰高及其比值在CHO分子结构的光谱剖面上发生了显著变化。Pearson分析结果表明木质素峰面积、纤维素复合面积、总CHO峰面积和CHO营养成分之间存在较强的相关性，但能量值除外（3倍维持摄食时的哺乳净能、维持净能和增重净能）。混合样品中STCHO峰面积与CHO营养谱无相关性。
The objectives of this study were to (1) differentiate mixtures of a co-product blend (70:30% mixture of wheat:corn before bioethanol fermentation) from bioethanol processing with barley grain based on Fourier transform infrared attenuated total reflection molecular spectroscopy with cluster analysis, (2) elucidate the effects of the co-product blend on the molecular structure spectral profile and nutritive characteristics of the hull-less barley carbohydrate (CHO) in dairy cattle, and (3) quantify the relationship between the CHO molecular structure spectral profiles and CHO nutritive characteristics of the mixtures in dairy cattle. The parameters assessed for CHO molecular structure spectral profiles included molecular spectral peak area and height intensities of lignin, structural CHO (STCHO), cellulosic compounds, and total CHO. The parameters assessed for CHO nutritive characteristics included the CHO nutrient profiles, CHO subfractions, energy values, and rumen-fermentable organic matter supply. Hull-less barley grains and the co-product blend were mixed according to the ratio of 100:0 (B100), 75:25, 50:50, 25:75, and 0:100 (BD100). The results showed that when the co-product blend was included at the different ratios, the predicted rumen-fermentable organic matter supply in the rumen was significantly decreased from 632 (B100) to 518 g/kg of dry matter (DM; BD100). The effective degradable dry matter in the rumen and the effective degradable starch in the rumen were also significantly decreased from 672 and 482 g/kg of DM (B100) to 475 and 43 g/kg of DM (BD100), respectively. By using Fourier transform infrared attenuated total reflection spectroscopy, we identified were significant changes in the spectral profiles of the CHO molecular structure in terms of lignin, STCHO, cellulosic compounds, and total CHO spectral peak area and height and their ratios. Pearson analysis results suggested strong correlations between lignin peak area, cellulosic compound area, and total CHO peak area and CHO nutrient profiles except energy values (net energy for lactation at 3 times maintenance intake, net energy for maintenance, and net energy for gain). No correlation was observed between STCHO peak area and CHO nutrient profiles in the mixture samples.