A murine autoimmune model of rheumatoid arthritis and systemic lupus erythematosus associated with deregulated production of IL-17 and IL-21.
A murine autoimmune model of rheumatoid arthritis and systemic lupus erythematosus associated with deregulated production of IL-17 and IL-21.
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DOI：
10.1007/978-1-60761-720-4_11
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Pernis, Alessandra B
中科院分区：
文献类型：
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作者：
Biswas, Partha S;Kang, Kyuho;Pernis, Alessandra B
辅助性T细胞17（Th 17）在包括风湿性关节炎（RA）和系统性红斑狼疮（SLE）在内的许多自身免疫性疾病的发病机制中起重要作用。在这一章中，我们描述了一个小鼠模型，其中IL-17和IL-21的生产失调可能导致狼疮样疾病或RA样症状，这取决于遗传背景。我们描绘的关键技术，可用于解剖负责这些疾病的发病机制，在细胞和分子水平，包括在体外Th 17细胞分化，染色质免疫沉淀试验，和逆转录病毒转导实验。我们还描述了可用于监测RA和SLE在小鼠模型中的经典临床结果的方法。鉴于IL-17和IL-21的失调产生在自身免疫中的广泛参与，这些技术中的许多对于在其他自身免疫疾病的鼠模型中研究这些途径也可能是有价值的。
T-helper cell 17 (Th17) cells play an important role in the pathogenesis of many autoimmune disorders including Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE). In this chapter we describe a murine model where deregulated production of IL-17 and IL-21 can lead to either lupus-like disease or RA-like symptoms depending on the genetic background. We delineate the key techniques that can be used to dissect the mechanisms responsible for the pathogenesis of these diseases at both a cellular and molecular level including in vitro Th17 cell differentiation, chromatin immunoprecipitation assays, and retroviral transduction experiments. We also describe the methodologies that can be utilized to monitor the classic clinical findings of RA and SLE in murine models. Given the broad involvement of deregulated production of IL-17 and IL-21 in autoimmunity, many of these techniques could also be valuable for the investigation of these pathways in murine models of other autoimmune diseases.