Activities and Properties of Peptidylarginine Deiminases of Several Vertebrate Brains
Activities and Properties of Peptidylarginine Deiminases of Several Vertebrate Brains
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几种脊椎动物脑肽基精氨酸脱亚氨酶的活性和特性
DOI：
10.1271/bbb1961.50.1303
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
K. Sugawara
中科院分区：
文献类型：
--
作者：
H. Takahara;K. Sueyoshi;K. Sugawara
对小鼠、大鼠、兔、猪、鸡和鸭的大脑提取物进行了肽基精氨酸脱亚胺酶的检测，该酶催化精氨酰残基的脱亚胺化。我们测试的所有大脑的提取物中都含有肽基精氨酸脱亚胺酶，这表明这种酶在脊椎动物大脑中广泛存在。 8种不同近交系小鼠的大脑活动水平可分为两组。高组和低组之间的差异超过300%。此外，从小鼠、猪和鸡的大脑中纯化的肽基精氨酸脱亚胺酶表明，它们的分子量和最佳活性条件没有太大差异。然而，鸡脑酶的底物特异性与哺乳动物脑酶的底物特异性有些不同。
Extracts from brains of mouse, rat, rabbit, pig, chicken, and duck were assayed for peptidylarginine deiminase, which catalyzes the deimination of arginyl residues. There was peptidylarginine deiminase in the extracts from all the brains that we tested, suggesting the widespread occurrence of this enzyme in vertebrate brains. The levels of the activity in the brains of 8 different inbred strains of mice could be divided into two groups. There is a difference of over 300% between the high and low groups. Furthermore peptidylarginine deiminase purified from the brains of mouse, pig, and chicken showed that there is no great difference in their MW and the optimal conditions for the activity. However, the substrate specificity of the enzyme from chicken brain was somewhat different from those of the enzymes from mammalian brains.