POLYMETHYLMETHACRYLATE PARTICLES ENHANCE DNA AND PROTEIN-SYNTHESIS OF HUMAN FIBROBLASTS INVITRO
POLYMETHYLMETHACRYLATE PARTICLES ENHANCE DNA AND PROTEIN-SYNTHESIS OF HUMAN FIBROBLASTS INVITRO
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DOI：
10.1002/jbm.820270508
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发表时间：
1993-05-01
期刊：
影响因子：
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通讯作者：
HUNGERFORD, DS
中科院分区：
文献类型：
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作者：
FRONDOZA, CG;TANNER, KT;HUNGERFORD, DS
聚甲基丙烯酸甲酯（PMMA）固定假体的无菌性松动的特征是形成放射性纤维膜、炎性细胞积聚和骨质溶解。由于这种膜是由成纤维细胞产生的，因此这些结缔组织细胞可能在松动过程中起关键作用。成纤维细胞是否在PMMA刺激下形成放射性骨水泥膜尚未确定，也不知道成纤维细胞是否通过分泌化学介质将炎性细胞吸引到骨-骨水泥界面。为了解决这个问题，我们分析了正常人成纤维细胞对PMMA的体外反应。将细胞用10（5）/mL Dulbecco基本必需培养基铺板，并在4小时后与PMMA颗粒、聚苯乙烯（PS）颗粒或单独的培养基孵育。通过掺入H-3-胸苷监测的降解能力在暴露于PMMA 48 h后显著增加。通过C-14-亮氨酸和C-14-脯氨酸的掺入确定的蛋白质合成也增加。相比之下，分泌的前列腺素（PG）E2免疫酶法测定的水平没有改变PMMA。暴露于对照PS的成纤维细胞没有改变其增殖或蛋白质合成活性。成纤维细胞内化PMMA和PS颗粒而没有可检测的超微结构损伤。在我们的体外试验系统中观察到PMMA增强成纤维细胞增殖能力和蛋白质合成能力，表明对体内成纤维细胞有类似的作用。
Aseptic loosening of polymethylmethacrylate (PMMA) fixed prosthesis is characterized by formation of a radiolucent fibrous membrane, accumulation of inflammatory cells and osteolysis. Since this membrane is produced by fibroblasts, it is likely that these connective tissue cells play a critical role in the loosening process. Whether fibroblasts form the radiolucent membrane in response to stimulation by PMMA has not yet been established, nor is it known whether fibroblasts play a role in attracting inflammatory cells to the bone-cement interface by secreting chemical mediators. To address this question, we analyzed the in vitro response of normal human fibroblasts to PMMA. Cells were plated with 10(5)/mL Dulbecco minimal essential medium and were incubated 4 h later with PMMA particles, polystyrene (PS) particles, or medium alone. Proliferative capacity monitored by incorporation of H-3-thymidine was significantly increased following a 48 h exposure to PMMA. Protein synthesis determined by incorporation of C-14-leucine and C-14-proline was also increased. In contrast, levels of secreted prostaglandin (PG) E2 assayed immunoenzymatically was not altered by PMMA. Fibroblasts exposed to control PS did not change their proliferative or protein synthetic activity. Fibroblasts internalized PMMA and PS particles without detectable ultrastructural damage. PMMA enhancement of fibroblast proliferative capacity and protein synthetic ability observed in our in vitro assay system suggests similar effects on fibroblasts in vivo.