Detection of canine distemper virus in dogs by real-time RT-PCR
Detection of canine distemper virus in dogs by real-time RT-PCR
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DOI：
10.1016/j.jviromet.2006.05.004
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发表时间：
2006-09-01
影响因子：
3.1
通讯作者：
Buonavoglia, Canio
中科院分区：
文献类型：
--
作者：
Elia, Gabriella;Decaro, Nicola;Buonavoglia, Canio
犬瘟热病毒是狗和许多其他食肉动物严重疾病的病原体。犬瘟热的临床诊断很困难，因为其症状广泛，可能与犬的其他呼吸道和肠道疾病混淆。因此，疑似病例需要实验室确认。在本研究中，开发了一种实时 RT-PCR 检测方法，用于犬瘟热病毒的检测和定量。该测定显示出高特异性，因为所有阴性对照（无模板对照和来自健康血清阴性狗的样本）和其他犬病原体都没有被误检。 TaqMan 检测可检测到多达 1 x 10(2) 个 RNA 拷贝，因此具有高灵敏度。定量 TaqMan 在临床样本上进行了验证，包括从自然感染犬瘟热病毒的狗身上采集的各种组织和器官。尿液、扁桃体、结膜拭子和全血被发现含有高病毒载量，因此被证明是检测犬瘟热病毒RNA的合适目标。 (c) 2006 Elsevier B.V. 保留所有权利。
Canine distemper virus is the etiological agent of a severe disease in dogs and many other carnivores. Clinical diagnosis of canine distemper is difficult due to the broad spectrum of signs that may be confounded with other respiratory and enteric diseases of dogs. Accordingly, a laboratory confirmation is required for suspected cases. In this study a real-time RT-PCR assay was developed for detection and quantitation of canine distemper virus. The assay exhibited high specificity as all the negative controls (no-template-controls and samples from healthy sero-negative dogs) and other canine pathogens were not misdetected. Up to 1 x 10(2) copies of RNA were detected by the TaqMan assay, thus revealing a high sensitivity. Quantitative TaqMan was validated on clinical samples, including various tissues and organs collected from dogs naturally infected by canine distemper virus. Urines, tonsil, conjunctival swabs and whole blood were found to contain high virus loads and therefore proved to be suitable targets for detection of canine distemper virus RNA. (c) 2006 Elsevier B.V. All rights reserved.