Laboratory selection for and characteristics of pyrethroid resistance in the malaria vector Anopheles funestus
Laboratory selection for and characteristics of pyrethroid resistance in the malaria vector Anopheles funestus
复制标题
DOI：
10.1111/j.1365-2915.2005.00574.x
复制
发表时间：
2005-09-01
影响因子：
1.9
通讯作者：
Coetzee, M
中科院分区：
文献类型：
--
作者：
Hunt, RH;Brooke, BD;Coetzee, M
2000年，从莫桑比克南部收集的材料中建立了一个实验室集落的按蚊funestus Giles（双翅目：蚊科），该地区的野生种群已证明对拟除虫菊酯具有抗性。使用0.1%三氟氯氰菊酯选择殖民地的子样本用于拟除虫菊酯抗性。在后续世代F-2至F-4中的生物测定敏感性测试显示，每一个连续世代的抗性增加。个别蚊子的生存只喂养10%的糖溶液，随着年龄的增加，最多4天，但到第10天已显着下降。然而，已经交配并给予血餐的雌性动物的死亡率并没有随着年龄的增长而增加。抗性个体和敏感个体的生化分析显示单加氧酶和谷胱甘肽S-转移酶活性增加，但与年龄无关。
A laboratory colony of Anopheles funestus Giles (Diptera: Culicidae) was established in 2000 from material collected from southern Mozambique where pyrethroid resistance had been demonstrated in the wild population. A subsample of the colony was selected for pyrethroid resistance using 0.1% lambda-cyhalothrin. Bioassay susceptibility tests in subsequent generations F-2 to F-4 showed increased resistance with each successive generation. Survival of individual mosquitoes fed only on 10% sugar solution, increased with age up to 4 days, but by day 10 had decreased significantly. However, females that had been mated and given bloodmeals showed no such increase in mortality with age. Biochemical analysis of resistant and susceptible individuals showed increased monooxygenase and glutathione S-transferase activity but no significant correlation with age of the mosquitoes.