Identification of long non-coding RNAs GAS5, linc0597 and lnc-DC in plasma as novel biomarkers for systemic lupus erythematosus.
Identification of long non-coding RNAs GAS5, linc0597 and lnc-DC in plasma as novel biomarkers for systemic lupus erythematosus.
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血浆中长非编码 RNA GAS5、linc0597 和 lnc-DC 的鉴定作为系统性红斑狼疮的新型生物标志物
DOI：
10.18632/oncotarget.15569
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发表时间：
2017-04-04
期刊：
影响因子：
--
通讯作者：
Ye DQ
中科院分区：
文献类型：
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作者：
Wu GC;Li J;Leng RX;Li XP;Li XM;Wang DG;Pan HF;Ye DQ
尽管越来越多的证据表明长非编码RNA(LncRNAs)广泛参与人类疾病，但lncRNAs在系统性红斑狼疮(SLE)中的作用尚不清楚。在这项研究中，我们进行了两个阶段的研究，以探讨五种lncRNAs(Gas5、linc0949、linc0597、HOTAIRM1和lnc-DC)的血浆水平及其作为SLE生物标志物的可能性。与健康对照组比较，系统性红斑狼疮患者血浆Gas5和LNC-DC水平显著降低(P<；0.001和P=0.002)，而LINC0597则过度表达(P<；0.001)。将SLE患者分为非肾炎型和狼疮性肾炎(LN)时，LN组LNC-DC水平显著高于非肾炎组(P=0.018)，而LN和LINC0597水平无明显差异，血浆LINC0949水平在所有比较中均无显著差异。对可能的生物标志物的进一步评价显示，Gas5、linc0597和lnc-DC可特异性地鉴别SLE患者，Gas5和linc0597联合检测可提供更好的诊断准确性，lnc-DC可区分LN和无肾炎的SLE。综上所述，血浆中的Gas5、linc0597和lnc-DC可能是SLE的潜在生物标志物。
Despite increasing evidence that long non-coding RNAs (lncRNAs) widely take part in human diseases, the role of lncRNAs in systemic lupus erythematosus (SLE) is largely unknown. In this study, we performed a two-stage study to explore the plasma levels of five lncRNAs (GAS5, linc0949, linc0597, HOTAIRM1 and lnc-DC) and their potential as SLE biomarkers. Compared with healthy controls, plasma levels of GAS5 and lnc-DC were significantly decreased (P < 0.001 and P = 0.002, respectively) while linc0597 were overexpressed in SLE patients (P < 0.001). When SLE patients were divided into SLE without nephritis and lupus nephritis (LN), the levels of lnc-DC were significantly higher in LN compared with SLE without nephritis (P = 0.018), but no significant difference in levels of GAS5 and linc0597 were found between LN and SLE without nephritis; plasma linc0949 level showed no significant difference in all comparisons. Further evaluation on potential biomarkers showed that GAS5, linc0597 and lnc-DC may specifically identify patients with SLE, the combination of GAS5 and linc0597 provided better diagnostic accuracy; lnc-DC may discriminate LN from SLE without nephritis. In summary, GAS5, linc0597 and lnc-DC in plasma could be potential biomarkers for SLE.