The Time Course of Long-Distance Signaling in Radiation-Induced Bystander Effect In Vivo in Arabidopsis thaliana Demonstrated Using Root Micro-Grafting
The Time Course of Long-Distance Signaling in Radiation-Induced Bystander Effect In Vivo in Arabidopsis thaliana Demonstrated Using Root Micro-Grafting
复制标题
使用根微嫁接证明了拟南芥体内辐射诱导的旁观者效应中长距离信号传导的时间过程
DOI：
10.1667/rr2486.1
复制
发表时间：
2011-08-01
影响因子：
3.4
通讯作者：
Yu, Zengliang
中科院分区：
文献类型：
--
作者：
Wang, Ting;Li, Fanghua;Yu, Zengliang
王涛，李峰，徐松，边鹏，吴艳，吴磊，于忠，于忠。基于根微嫁接的拟南芥辐射诱导旁观者效应远距离信号的时间过程研究。Radiat。Res. 176, 234-243（2011）。辐射诱导的旁观者效应已在整个生物体以及体外多细胞组织和体外单细胞培养系统中得到证实。然而，旁观者信号的时间过程，特别是在整个生物体中，尚不清楚。我们的研究小组已经证明了植物体内的远距离旁观者/抽象效应。嫁接是研究植物根冠信号转导的有效实验手段。在本研究中，我们开发了一种拟南芥幼苗的根微嫁接技术，该技术可以在根辐照后的特定时间很容易地停止或启动根与地上部的旁观者信号通信。使用这种方法，我们以AtRAD54基因的表达水平作为生物学终点，在生物体水平上展示了辐射诱导旁观者效应中长距离信号传导的时间过程。简而言之，照射后旁观者部位损伤信号的8小时积累对于引发旁观者反应至关重要。信号积累的延长与信号分子在植物体内的传递速度无关，也不是由于靶根细胞中旁观者信号的延迟启动所致。认为旁观者效应可能是由辐照后不同阶段启动的多个旁观者信号共同诱导的。此外，活性氧（ROS）被证明与旁观者细胞对辐射损伤信号的反应过程有关，而不是与靶细胞中旁观者信号的产生有关。(C)辐射研究学会2011
Wang, T., Li, F., Xu, S., Bian, P., Wu, Y., Wu, L. and Yu, Z. The Time Course of Long-Distance Signaling in Radiation-Induced Bystander Effect In Vivo in Arabidopsis thaliana Demonstrated Using Root Micro-Grafting. Radiat. Res. 176, 234-243 (2011).The radiation-induced bystander effect has been demonstrated in whole organisms as well as in multicellular tissues in vitro and single-cell culture systems in vitro. However, the time course of bystander signaling, especially in whole organisms, is not clear. Long-distance bystander/abscopal effects in vivo in plants have been demonstrated by our group. Plant grafting is a useful experimental tool for studying the root-shoot signaling of plants. In the present study, we developed a root micro-grafting technique with young seedlings of Arabidopsis thaliana in which the bystander signaling communication of root-to-shoot could easily be stopped or started at specific times after root irradiation. Using this methodology, we demonstrated the time course of long-distance signaling in radiation-induced bystander effects at the level of the organism using the expression level of the AtRAD54 gene as a biological end point. Briefly, an 8-h accumulation of damage signals in bystander parts after irradiation was essential for eliciting a bystander response. The protraction of signal accumulation was not related to the transmission speed of signaling molecules in plants and did not result from the delayed initiation of bystander signals in targeted root cells. It was suggested that the bystander effect might be induced jointly by multiple bystander signals initiated at different stages after irradiation. Moreover, reactive oxygen species (ROS) were shown to be implicated in the response process of bystander cells to radiation damage signals rather than in the generation of bystander signals in targeted cells. (C) 2011 by Radiation Research Society