A role for PM19-Like 1 in seed dormancy in Arabidopsis
A role for PM19-Like 1 in seed dormancy in Arabidopsis
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DOI：
10.1017/s0960258519000151
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发表时间：
2019-09-01
影响因子：
2.1
通讯作者：
Gubler, Frank
中科院分区：
文献类型：
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作者：
Barrero, Jose M.;Dorr, Marie M.;Gubler, Frank
在过去的几年里，人们对小麦种子休眠的遗传基础的理解得到了迅速的提高，并且已经鉴定了许多与该性状相关的基因。我们最近发现了小麦基因TaPM 19-A1和-A2，我们现在已经迈出了理解这类基因在种子中作用的第一步。通过研究拟南芥同源PM 19样1（PM 19 L1），我们发现，它有一个种子特异性的表达模式，而它的表达是在休眠种子比在非休眠种子，敲除突变产生的种子增加休眠。功能丧失不仅增加了初休眠，而且增加了对高温反应的次生休眠。我们还通过将PM 19蛋白主要定位于种子中的子叶细胞（可能在膜中）来研究PM 19 L1的功能。通过研究该基因的共表达网络，我们发现它与脱落酸（阿坝）诱导的种子成熟和贮藏相关基因的一个小团体。PM 19 L1的功能为探讨阿坝、温度和膜特性等影响种子休眠的关键因子之间的相互作用提供了一个很好的机会。
The understanding of the genetic basis of grain dormancy in wheat has rapidly improved in the last few years, and a number of genes have been identified related to that trait. We recently identified the wheat genes TaPM19-A1 and -A2 and we have now taken the first step towards understanding the role of this class of genes in seeds. By investigating the Arabidopsis homologous PM19-Like 1 (PM19L1) we have found that it has a seed-specific expression pattern and, while its expression is higher in dormant than in non-dormant seeds, knock-out mutations produced seeds with increased dormancy. Not only primary dormancy, but also secondary dormancy in response to high temperature was increased by the loss-of-function. We have also examined the function of PM19L1 by localizing the PM19 protein primarily to the cotyledon cells in seeds, possibly in membranes. By investigating the co-expression network of this gene we have found that it is connected to a small group of abscisic acid (ABA)-induced seed maturation and storage-related genes. The function of PM19L1 represents a good opportunity to explore the interactions of key factors that can influence seed dormancy such as ABA, temperature and membrane properties.