Introns regulate RNA and protein abundance in yeast
Introns regulate RNA and protein abundance in yeast
复制标题
DOI：
10.1534/genetics.106.058560
复制
发表时间：
2006-09-01
期刊：
影响因子：
3.3
通讯作者：
Davis, Ronald W.
中科院分区：
文献类型：
--
作者：
Juneau, Kara;Miranda, Molly;Davis, Ronald W.
酿酒酵母结构简单基因组中内含子的作用还不是很清楚。为了分析内含子的功能相关性，对酿酒酵母内含子基因进行了一系列的计算分析和几个详细的缺失研究。从全基因组对酵母的研究中挖掘现有的数据，发现含有内含子的基因比非内含子基因产生更多的RNA和更多的蛋白质，而且更有可能是单倍体不足的。所有内含子基因的这些观察结果都适用于不同的基因亚群，包括核糖体、非核糖体、复制和非复制。与计算分析的结果相印证的是，三个基本基因内含子的缺失降低了细胞的RNA水平，并导致了可测量的生长缺陷。这些数据提供了证据，证明内含子提高了转录和翻译产量，是酵母竞争性生长所必需的。
The purpose of introns in the architecturally simple genome of Saccharomyces cerevisiae is not well understood. To assay the functional relevance of introns, a series of computational analyses and several detailed deletion studies were completed on the intronic genes of S. cerevisiae. Mining existing data from genomewide studies on yeast revealed that intron-containing genes produce more RNA and more protein and are more likely to be haplo-insufficient than nonintronic genes. These observations for all intronic genes held true for distinct subsets of genes including ribosomal, nonribosomal, duplicated, and nonduplicated. Corroborating the result of computational analyses, deletion of introns from three essential genes decreased cellular RNA levels and caused measurable growth defects. These data provide evidence that introns improve transcriptional and translational yield and are required for competitive growth of yeast.