Potentiation of myoblast transplantation by host muscle irradiation is dependent on the rate of radiation delivery
Potentiation of myoblast transplantation by host muscle irradiation is dependent on the rate of radiation delivery
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DOI：
10.1007/s004419900062
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发表时间：
1999-11-01
影响因子：
3.6
通讯作者：
Morgan, JE
中科院分区：
文献类型：
--
作者：
Gross, JG;Bou-Gharios, G;Morgan, JE
肌肉前体细胞移植(MPC)已被建议作为一种治疗肌肉疾病，如Duchenne肌营养不良症。16-20Gy射线照射骨骼肌可阻止肌肉再生，也可促进植入的肌肉前体细胞(MPC)形成肌肉。然而，当MDX nu/nu小鼠的肌肉在注射正常的MPC之前以0.73Gymin而不是1.29Gymin的预照射时，显著地形成了更多来自供体的肌肉纤维。这表明，照射的速度对肌肉有生理影响。虽然在植入MPC之前照射患者的肌肉是不可行的，但一旦确定了照射后肌肉中改变的因子(S)，就有可能利用这些因素来提高成肌细胞移植的成功率。
Transplantation of muscle precursor cells (mpc) has been suggested as a treatment for myopathies, such as Duchenne muscular dystrophy. Irradiation of skeletal muscle with 16-20 Gy prevents muscle regeneration and also augments muscle formation from implanted muscle precursor cells (mpc). However, when mdx nu/nu mouse muscles are preirradiated at 0.73 Gy/min rather than at 1.29 Gy/min prior to their injection with normal mpc, significantly more muscle fibres of donor origin are formed. This suggests that the rate at which irradiation is delivered has a physiological effect on the muscle. Although it would not be feasible to irradiate a patient's muscles prior to mpc implantation, once the factor(s) which are altered in irradiated muscle have been identified, it might be possible to use these to increase the success of myoblast transplantation.