Soil exposure modifies the gut microbiota and supports immune tolerance in a mouse model
Soil exposure modifies the gut microbiota and supports immune tolerance in a mouse model
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DOI：
10.1016/j.jaci.2018.06.024
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发表时间：
2019-03-01
影响因子：
14.2
通讯作者：
Fyhrquist, Nanna
中科院分区：
文献类型：
--
作者：
Ottman, Noora;Ruokolainen, Lasse;Fyhrquist, Nanna
背景资料：充分暴露于自然环境，特别是土壤及其微生物，已被认为是对过敏的保护作用。目的：我们旨在通过研究暴露于土壤后小鼠肠道微生物群的定殖以及通过检查稳态情况和过敏性炎症期间的免疫状态来获得环境-微生物群-健康轴的更直接证据。方法：通过使用16 S rRNA基因测序分析了饲养在干净床上或与土壤接触的小鼠的胃肠道微生物群，并将数据与肠道粘膜、肺组织和血清样品中测量的免疫参数相结合。我们观察到在干净的床上或与土壤接触的小鼠之间的小肠和粪便微生物群组成的显着差异，在土壤组中，拟杆菌门相对于厚壁菌门的比例更高。住房环境也影响小鼠肠道基因的表达，如所示的上调表达的免疫调节标志物IL-10，叉头盒P3，和细胞毒性T淋巴细胞相关蛋白4的土壤组。重要的是，使用小鼠哮喘模型，我们发现暴露于土壤使免疫系统向T（H）1和更高水平的抗炎信号极化，减轻T（H）2型过敏反应。小鼠的炎症状态有显着的影响肠道菌群的组成，表明双向通信沿着肠-肺axills.Conclusion：我们的研究结果提供了证据的作用，环境获得性微生物在减轻对T（H）2驱动的炎症，这涉及到过敏性疾病。
Background: Sufficient exposure to natural environments, in particular soil and its microbes, has been suggested to be protective against allergies.Objective: We aim at gaining more direct evidence of the environment-microbiota-health axis by studying the colonization of gut microbiota in mice after exposure to soil and by examining immune status in both a steady-state situation and during allergic inflammation.Methods: The gastrointestinal microbiota of mice housed on clean bedding or in contact with soil was analyzed by using 16S rRNA gene sequencing, and the data were combined with immune parameters measured in the gut mucosa, lung tissue, and serum samples.Results: We observed marked differences in the small intestinal and fecal microbiota composition between mice housed on clean bedding or in contact with soil, with a higher proportion of Bacteroidetes relative to Firmicutes in the soil group. The housing environment also influenced mouse intestinal gene expression, as shown by upregulated expression of the immunoregulatory markers IL-10, forkhead box P3, and cytotoxic T lymphocyte-associated protein 4 in the soil group. Importantly, using the murine asthma model, we found that exposure to soil polarizes the immune system toward T(H)1 and a higher level of anti-inflammatory signaling, alleviating T(H)2-ype allergic responses. The inflammatory status of the mice had a marked influence on the composition of the gut microbiota, suggesting bidirectional communication along the gut-lung axis.Conclusion: Our results provide evidence of the role of environmentally acquired microbes in alleviating against T(H)2-driven inflammation, which relates to allergic diseases.