High-Speed Atomic Force Microscopy Reveals Spontaneous Nucleosome Sliding of H2A.Z at the Subsecond Time Scale
High-Speed Atomic Force Microscopy Reveals Spontaneous Nucleosome Sliding of H2A.Z at the Subsecond Time Scale
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高速原子力显微镜揭示了 H2A.Z 在亚秒级时间尺度的自发核小体滑动
DOI：
10.1021/acs.nanolett.2c04346
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发表时间：
2023
期刊：
影响因子：
10.8
通讯作者：
Shibata Mikihiro
中科院分区：
文献类型：
--
作者：
Morioka Shin;Sato Shoko;Horikoshi Naoki;Kujirai Tomoya;Tomita Takuya;Baba Yudai;Kakuta Takahiro;Ogoshi Tomoki;Puppulin Leonardo;Sumino Ayumi;Umeda Kenichi;Kodera Noriyuki;Kurumizaka Hitoshi;Shibata Mikihiro
核小体动力学，如核小体滑动和DNA展开，在真核染色质的基因调控中是重要的。H2A。Z是组蛋白H2A的一种高度进化保守的变体，通过在体内和体外形成不稳定的多位置核小体蛋白参与基因调控。然而，这种不稳定核小体的亚秒动力学还没有在生理条件下直接可视化。在这里，我们使用高速原子力显微镜（HS-AFM）直接观察人类h2a . z .1核小体的亚秒动力学。HS-AFM视频显示核小体在0.3秒内沿着4 nm的DNA向任何方向滑动。这种滑动也可以在haa中看到。突变体中，c端一半被相应的规范H2A氨基酸所取代，表明H2A的n端区域相互作用。DNA负责核小体的滑动。这些结果可能揭示了核小体动力学与组蛋白H2A.Z基因调控的关系。
Nucleosome dynamics, such as nucleosome sliding and DNA unwrapping, are important for gene regulation in eukaryotic chromatin. H2A.Z, a variant of histone H2A that is highly evolutionarily conserved, participates in gene regulation by forming unstable multipositioned nucleosomesin vivoandin vitro. However, the subsecond dynamics of this unstable nucleosome have not been directly visualized under physiological conditions. Here, we used high-speed atomic force microscopy (HS-AFM) to directly visualize the subsecond dynamics of human H2A.Z.1-nucleosomes. HS-AFM videos show nucleosome sliding along 4 nm of DNA within 0.3 s in any direction. This sliding was also visualized in an H2A.Z.1 mutant, in which the C-terminal half was replaced by the corresponding canonical H2A amino acids, indicating that the interaction between the N-terminal region of H2A.Z.1 and the DNA is responsible for nucleosome sliding. These results may reveal the relationship between nucleosome dynamics and gene regulation by histone H2A.Z.