An RNA-interacting protein, SYNCRIP (heterogeneous nuclear ribonuclear protein Q1/NSAP1) is a component of mRNA granule transported with inositol 1,4,5-trisphosphate receptor type 1 mRNA in neuronal dendrites
An RNA-interacting protein, SYNCRIP (heterogeneous nuclear ribonuclear protein Q1/NSAP1) is a component of mRNA granule transported with inositol 1,4,5-trisphosphate receptor type 1 mRNA in neuronal dendrites
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DOI：
10.1074/jbc.m409732200
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发表时间：
2004-12-17
影响因子：
4.8
通讯作者：
Mikoshiba, K
中科院分区：
文献类型：
--
作者：
Bannai, H;Fukatsu, K;Mikoshiba, K
神经元树突的mRNA转运和局部翻译与突触可塑性的诱导有关。最近，我们克隆了一个rna相互作用蛋白SYNCRIP（异质核核糖核蛋白Q1/NSAP1），该蛋白被认为对mRNA的稳定很重要。我们在这里报道SYNCRIP是大鼠海马神经元mRNA颗粒的一个组成部分。SYNCRIP主要在细胞体中表达，但在近端树突中也可见点状表达模式。活体神经元的延时分析显示，用荧光蛋白标记的SYNCRIP标记的颗粒在树突内以类似于0.05妈妈/秒的速度双向运输。用诺可达唑处理神经元可显著抑制绿色荧光蛋白- syncrip阳性颗粒的运动，表明含syncrip颗粒的运输依赖于微管。含有syn - crip的颗粒的分布与树突rna和延伸因子1α的分布重叠。SYNCRIP还被发现与绿色荧光蛋白标记的人staufen1和肌醇1,4,5-三磷酸受体1型mRNA的3'-未翻译区共转运。这些结果表明SYNCRIP作为mRNA颗粒的组成部分在树突内运输，并提出了mRNA颗粒中mRNA的转换和神经元树突局部蛋白质合成的调节可能涉及SYNCRIP。
mRNA transport and local translation in the neuronal dendrite is implicated in the induction of synaptic plasticity. Recently, we cloned an RNA-interacting protein, SYNCRIP ( heterogeneous nuclear ribonuclear protein Q1/NSAP1), that is suggested to be important for the stabilization of mRNA. We report here that SYNCRIP is a component of mRNA granules in rat hippocampal neurons. SYNCRIP was mainly found at cell bodies, but punctate expression patterns in the proximal dendrite were also seen. Time-lapse analysis in living neurons revealed that the granules labeled with fluorescent protein-tagged SYNCRIP were transported bi-directionally within the dendrite at similar to0.05 mum/s. Treatment of neurons with nocodazole significantly inhibited the movement of green fluorescent protein-SYNCRIP-positive granules, indicating that the transport of SYNCRIP-containing granules is dependent on microtubules. The distribution of SYN-CRIP-containing granules overlapped with that of dendritic RNAs and elongation factor 1alpha. SYNCRIP was also found to be co-transported with green fluorescent protein-tagged human staufen1 and the 3'-untranslated region of inositol 1,4,5-trisphosphate receptor type 1 mRNA. These results suggest that SYNCRIP is transported within the dendrite as a component of mRNA granules and raise the possibility that mRNA turnover in mRNA granules and the regulation of local protein synthesis in neuronal dendrites may involve SYNCRIP.