TISSUE-SPECIFIC EXPRESSION, DEVELOPMENTAL REGULATION, AND GENETIC-MAPPING OF THE GENE ENCODING CCAAT ENHANCER BINDING-PROTEIN
TISSUE-SPECIFIC EXPRESSION, DEVELOPMENTAL REGULATION, AND GENETIC-MAPPING OF THE GENE ENCODING CCAAT ENHANCER BINDING-PROTEIN
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DOI：
10.1101/gad.3.8.1146
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发表时间：
1989-08-01
影响因子：
10.5
通讯作者：
MCKNIGHT, SL
中科院分区：
文献类型：
--
作者：
BIRKENMEIER, EH;GWYNN, B;MCKNIGHT, SL
本文介绍了实验的结果，确定的小鼠基因编码CCAAT/增强子结合蛋白（C/EBP）的染色体定位，并测量其表达的功能，在小鼠和大鼠的组织类型和时间的发展。根据限制性片段长度多态性鉴定了C/EBP基因的三个等位基因。测定了这三个等位基因在重组近交系小鼠中的品系分布，并与其它小鼠基因的品系分布进行了比较。这些结果将该基因定位到小鼠7号染色体上结构基因葡萄糖磷酸异构酶的2.5厘摩（cM）内的位置。通过核酸杂交和抗体染色分析的组合，C/EBP基因的表达模式进行了研究。在已知以不常见的高速率代谢脂质和胆固醇相关化合物的组织中观察到高水平的C/EBP mRNA。这些包括肝脏、脂肪、肠、肺、肾上腺和胎盘。对这些组织中的两种（肝脏和脂肪）进行更详细的分析表明，C/EBP表达仅限于完全分化的细胞。此外，在两个组织，肝脏和肠道的C/EBP mRNA的表达的时间模式的分析，揭示了一个协调的出生前。这些观察结果提出了这样的可能性，即C/EBP的合成可能对体液因素有反应，并且C/EBP表达的调节可能介导基因表达的协调变化，从而促进在发育期间或在成年生活的波动生理状态期间遇到的适应性挑战。
This paper presents the results of experiments that determine the chromosomal location of the mouse gene encoding CCAAT/enhancer binding protein (C/EBP) and measure its expression as a function of tissue type and temporal period of development in mice and rats. Three alleles of the C/EBP gene were identified according to restriction fragment length polymorphisms. The strain distibution pattern of the three alleles was determined in recombinant inbred mouse strains and compared to that of other mouse genes. These results mapped the gene to a position within 2.5 centimorgans (cM) of the structural gene glucose phosphate isomerase on chromosome 7 of the mouse. The expression pattern of the C/EBP gene was studied by a combination of nucleic acid hybridization and antibody staining assays. High levels of C/EBP mRNA were observed in tissues known to metabolize lipid and cholesterol-related compounds at uncommonly high rates. These included liver, fat, intestine, lung, adrenal gland, and placenta. More detailed analysis of two of these tissues, liver and fat, showed that C/EBP expression was limited to fully differentiated cells. Moreover, analysis of the temporal pattern of expression of C/EBP mRNA in two tissues, liver and intestine, revealed a coordinated just prior to birth. These observations raise the possibility that the synthesis of C/EBP may be responsive to humoral factors and that modulation in C/EBP expression might mediate coordinated changes in gene expression that facilitate adaptive challenges met during development or during the fluctuating physiological states of adult life.