Physical mapping of chromosome 17 cosmids by fluorescence in situ hybridization and digital image analysis.
Physical mapping of chromosome 17 cosmids by fluorescence in situ hybridization and digital image analysis.
复制标题
通过荧光原位杂交和数字图像分析对 17 号染色体粘粒进行物理定位。
作者：
O. Kallioniemi;A. Kallioniemi;L. Mascio;D. Sudar;D. Pinkel;L. Deaven;J. Gray
我们使用荧光原位杂交和数字图像分析定位Cosmetry沿沿着人类17号染色体。从小鼠-人杂交细胞系的流式分选染色体的部分Sau 3AI消化物构建的17号染色体文库中随机选择71个克隆。其中63个（89%）只在17号染色体上发出信号。使用数字图像分析精确地绘制了在中期和间期细胞中产生最明显的杂交信号的40个cosmetics。另外20个cosmetics，以前映射的连锁分析，也被映射。这些探针的中期定位确定的顺序与连锁分析确定的顺序一致。
We used fluorescence in situ hybridization and digital image analysis to localize cosmids along human chromosome 17. Seventy-one cosmids were selected at random from a chromosome 17 library constructed from a partial Sau3AI digest of flow-sorted chromosomes from a mouse-human hybrid cell line. Sixty-three of these (89%) gave a signal only on chromosome 17. The 40 cosmids producing the most distinct hybridization signals in metaphase and interphase cells were precisely mapped using digital image analysis. An additional 20 cosmids, previously mapped by linkage analysis, were also mapped. The order of these probes determined by metaphase mapping was consistent with the order determined by linkage analysis.