THE DISTRIBUTION OF TRANSFERRIN IMMUNOREACTIVITY IN THE RAT CENTRAL-NERVOUS-SYSTEM
THE DISTRIBUTION OF TRANSFERRIN IMMUNOREACTIVITY IN THE RAT CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1016/0006-8993(86)90576-7
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发表时间：
1986-03-19
期刊：
影响因子：
2.9
通讯作者：
FINE, RE
中科院分区：
文献类型：
--
作者：
CONNOR, JR;FINE, RE
最近的研究表明，神经系统中存在转铁蛋白的受体，转铁蛋白是血液中的铁结合和运输蛋白。本研究使用免疫组织化学在光镜和电镜水平表明，转铁蛋白（Tf）主要是在大脑皮层，小脑和脊髓的灰色和白色物质的少突胶质细胞中发现。在大脑皮层内，第V层比其他皮层层具有更多的Tf标记细胞。在脊髓中，第VII层具有最高密度的Tf阳性细胞。根据位置，可以描述3种类型的少突胶质细胞：神经元周围、束间和血管周围。除少突胶质细胞外，第V层锥体细胞和前角细胞的内皮细胞和可能的一些神经元膜也标记有Tf抗血清。在超微结构上，Tf反应产物均匀分布在少突胶质细胞和内皮细胞的核周细胞质中。铁在运动和行为功能中的重要性已经得到了很好的确立，尽管铁在CNS中的作用机制还不清楚。Tf在少突胶质细胞中的存在意味着这些神经胶质细胞参与CNS中的铁动员和储存。铁的储存量和通过储存的转铁蛋白动员铁的能力可能是引起缺铁性CNS疾病所需的极端饮食限制的原因。
Recent studies have demonstrated receptors in the nervous system for transferrin, the iron binding and transport protein in the blood. This study using immunohistochemistry at the light and electron microscopic levels demonstrates that transferrin (Tf) is found predominantly in oligodendrocytes in both the gray and white matter of the cerebral cortex, cerebellum and spinal cord. Within the cerebral cortex, layer V has more Tf-labeled cells than the other cortical layers. In the spinal cord, lamina VII has the highest density of Tf-positive cells. Based on location, 3 types of oligodendrocytes can be described: perineuronal, interfascicular and perivascular. In addition to oligodendrocytes, endothelial cells and possibly some neuronal membranes of layer V pyramidal and anterior horn cells label with Tf antiserum. Ultrastructurally, Tf reaction product is homogeneously distributed throughout the perinuclear cytoplasm of both oligodendrocytes and endothelial cells. The importance of iron in motor and behavior function is well established although the mechanism of action of iron in the CNS is not well understood. The presence of Tf in oligodendrocytes implies that these neuroglia are involved in iron mobilization and storage in the CNS. Stored quantities of iron and the ability to mobilize the iron through stored transferrin may be the reason for the extreme dietary restrictions necessary to induce iron-deficient CNS disorders.