The acidic sequence of the NS4A cofactor regulates ATP hydrolysis by the HCV NS3 helicase.
The acidic sequence of the NS4A cofactor regulates ATP hydrolysis by the HCV NS3 helicase.
复制标题
NS4A 辅因子的酸性序列通过 HCV NS3 解旋酶调节 ATP 水解。
DOI：
10.1007/s00705-010-0838-2
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发表时间：
2011
影响因子：
2.7
通讯作者：
Strongin,AlexY
中科院分区：
文献类型：
--
作者：
Shiryaev,SergeyA;Chernov,AndreiV;Shiryaeva,TatianaN;Aleshin,AlexanderE;Strongin,AlexY
In flaviviruses and hepatitis C virus (HCV), the NS3 gene encodes the N-terminal protease (NS3pro) and the C-terminal helicase (NS3hel). In HCV, the downstream NS4A is required for the NS3pro activity and exhibits a conserved EFDEMEE motif. To identify the role of this motif, we compared the ATPase and helicase activities of NS3 alone with those of the NS3-NS4A constructs. Our results suggest that the EFDEMEE motif is essential for regulating the ATPase activity of NS3hel. It is likely that this motif interferes with the ATP-binding site of NS3hel. It is becoming clear that NS4A functions as a cofactor of both proteinase and helicase in HCV.
