Does the proliferation fraction help identify mature B cell lymphomas with double- and triple-hit translocations?
Does the proliferation fraction help identify mature B cell lymphomas with double- and triple-hit translocations?
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DOI：
10.1111/j.1365-2559.2012.04351.x
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发表时间：
2012-12-01
期刊：
影响因子：
6.4
通讯作者：
Tan, Soo Yong
中科院分区：
文献类型：
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作者：
Mationg-Kalaw, Emarene;Tan, Leonard H. C.;Tan, Soo Yong
Mationg-Kalaw E，Tan L H C，Tay K，Lim S T，Tang T，Lee Y Y L & Tan S Y？03 The Famous Women（2012）增殖分数是否有助于鉴别具有双重和三重易位的成熟B细胞淋巴瘤？目的：具有介于弥漫性大B细胞淋巴瘤（DLBCL）和伯基特淋巴瘤（BL）之间的特征的实体B细胞淋巴瘤（不可分类）是指具有BL和DLBCL重叠特征的B细胞肿瘤。这些灰区淋巴瘤的一个子集具有C-MYC和IGH易位，但除此之外，还包含BCL 2和/或BCL 6基因的额外重排。本研究的目的是调查增殖分数Ki67免疫染色可以用来识别这种双重/三重打击淋巴瘤。方法与结果：我们研究了492例成熟的侵袭性B细胞肿瘤的组织学，免疫组化和间期荧光原位杂交（FISH）使用分裂探针对C-MYC，BCL 2，BCL 6，IGH，MALT 1，PAX 5和CCND1。确定了40例伯基特淋巴瘤和28例MYC+双重/三重打击淋巴瘤。在后者中，77%和54%分别显示增殖分数超过75%和90%。Ki67免疫染色的截止值> 75%，检测MYC+双/三易位的灵敏度和特异性分别为0.77和0.36。将增殖分数标准提高到> 90%将特异性提高到0.62，但以0.54的低灵敏度为代价。结论：Ki 67的免疫染色不是一个有用的方法来预先筛选B细胞淋巴瘤MYC+双/三易位。
Mationg-Kalaw E, Tan L H C, Tay K, Lim S T, Tang T, Lee Y Y L & Tan S Y ?(2012) Histopathology?Does the proliferation fraction help identify mature B cell lymphomas with double- and triple-hit translocations? Aims: The entity B cell lymphoma, unclassifiable, with features intermediate between diffuse large B cell lymphoma (DLBCL) and Burkitt lymphoma (BL) refers to B cell neoplasms that share overlapping characteristics of BL and DLBCL. A subset of these grey-zone lymphomas possesses C-MYC and IGH translocations but, in addition, contains additional rearrangements of BCL2 and/or BCL6 genes. The aim of this study was to investigate if the proliferation fraction by Ki67 immunostaining can be used to identify such double-/triple-hit lymphomas. Methods and results: We studied 492 cases of mature aggressive B cell neoplasms by histology, immunohistochemistry and interphase fluorescence in-situ hybridization (FISH) using break-apart probes against C-MYC, BCL2, BCL6, IGH, MALT1, PAX5 and CCND1. Forty Burkitt lymphomas and 28 cases of MYC+ double-/triple-hit lymphomas were identified. Of the latter, 77% and 54% displayed proliferation fractions exceeding 75% and 90%, respectively. With a cut-off of >75% by Ki67 immunostaining, the sensitivity and specificity for detection of MYC+ double/triple translocations was 0.77 and 0.36. Raising the proliferation fraction criterion to >90% improved the specificity to 0.62 at the expense of a low sensitivity of 0.54. Conclusions: Immunostaining for Ki67 is not a useful approach to prescreen B cell lymphomas for MYC+ double/triple translocations.