Application of In Vivo EPR for Tissue pO2 and Redox Measurements
Application of In Vivo EPR for Tissue pO2 and Redox Measurements
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DOI：
10.1007/978-1-60327-017-5_10
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Das, Dipak K.
中科院分区：
文献类型：
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作者：
Khan, Nadeem;Das, Dipak K.
电子顺磁共振 (EPR) 波谱技术已有 50 多年的历史，但直到最近才被用于体内研究。过去其应用受到限制，是由于生物样品中的含水量较高，导致激发频率的非谐振介电损耗较高的问题。然而，随着过去 15 年中工作频率较低（1,200 MHz 及以下）的光谱仪的发展，现在可以对各种动物和离体器官进行活体测量。开发具有高灵敏度和适当稳定性的体内应用新型谐振器进一步促进了这一点。现在，利用体内 EPR 获得对生理学和病理生理学复杂方面的新见解已经变得可行。在该技术的几个重要应用中，病体内组织 pO(2)（氧分压）和氧化还原测量似乎是该技术最广泛的应用。在本章中，我们描述了动物模型中体内 pO(2) 和氧化还原测量的程序。
The technique of electron paramagnetic resonance (EPR) spectroscopy is more than 50 years old, but only recently it has been used for in vivo studies. Its limited application in the past was due to the problem of high nonresonant dielectric loss of the exciting frequency because of high water content in biological samples. However, with the development of spectrometers working at lower frequencies (1,200 MHz and below) during the last 15 years, it is now possible to conduct ill vivo measurements oil a variety of animals and isolated organs. This is further facilitated by the development of new resonators with high sensitivity and appropriate stability for in vivo applications. It now has become feasible to obtain new insights into the complex aspects of physiology and pathophysiology using in vivo EPR. Among several important applications of this technique, the ill Vivo tissue pO(2) (partial pressure of oxygen) and redox measurements seem to be the most extensive use of this technique. In this chapter, we describe the procedure for in vivo pO(2) and redox measurements in animal models.