Facing and Overcoming Sensitivity Challenges in Biomolecular NMR Spectroscopy.
Facing and Overcoming Sensitivity Challenges in Biomolecular NMR Spectroscopy.
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DOI：
10.1002/anie.201410653
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发表时间：
2015-08-03
期刊：
影响因子：
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通讯作者：
Frydman L
中科院分区：
文献类型：
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作者：
Ardenkjaer-Larsen JH;Boebinger GS;Comment A;Duckett S;Edison AS;Engelke F;Griesinger C;Griffin RG;Hilty C;Maeda H;Parigi G;Prisner T;Ravera E;van Bentum J;Vega S;Webb A;Luchinat C;Schwalbe H;Frydman L
2013年春，核磁共振光谱学家聚集在以色列魏茨曼研究所，集思广益，研究提高核磁共振实验灵敏度的方法，特别是在生物分子环境中应用时。这份多作者的跨学科综述对所考虑的主要方法进行了最先进的描述。讨论的主题包括超高场核磁共振系统的未来，新兴的核磁共振检测技术，核超极化的新方法，以及样品制备的进展。所有这些都是相互独立的努力，它们的成果可以成倍增加，从而提高固态和液态实验的灵敏度。虽然在所有这些领域都取得了实质性进展，但在寻求赋予核磁共振光谱以灵敏度的过程中仍然存在许多挑战，这种灵敏度是基于电学或光学测量的各种光谱分析的特征。这些挑战以及科学家和工程师正在努力解决这些挑战的方式也得到了解决。这篇综述介绍了当今正在考虑的提高核磁共振光谱灵敏度的主要方法的最新描述，特别是在生物分子环境中的应用。重点是超高场核磁共振系统的未来，新兴的核磁共振检测技术，核超极化的新方法，以及样品制备的进展。
In the Spring of 2013, NMR spectroscopists convened at the Weizmann Institute in Israel to brainstorm on approaches to improve the sensitivity of NMR experiments, particularly when applied in biomolecular settings. This multi-author interdisciplinary Review presents a state-of-the-art description of the primary approaches that were considered. Topics discussed included the future of ultrahigh-field NMR systems, emerging NMR detection technologies, new approaches to nuclear hyperpolarization, and progress in sample preparation. All of these are orthogonal efforts, whose gains could multiply and thereby enhance the sensitivity of solid- and liquid-state experiments. While substantial advances have been made in all these areas, numerous challenges remain in the quest of endowing NMR spectroscopy with the sensitivity that has characterized forms of spectroscopies based on electrical or optical measurements. These challenges, and the ways by which scientists and engineers are striving to solve them, are also addressed. This Review presents a state-of-the-art description of the leading approaches being considered today to improve the sensitivity of NMR spectroscopy, particularly as applied in biomolecular settings. The focus is on the future of ultrahigh-field NMR systems, emerging NMR detection technologies, new approaches to nuclear hyperpolarization, and progress in sample preparation.