PCR-RAFT: rapid high throughput oxygen tolerant RAFT polymer synthesis in a biology laboratory
PCR-RAFT: rapid high throughput oxygen tolerant RAFT polymer synthesis in a biology laboratory
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DOI：
10.1039/c9py01521c
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发表时间：
2020-02-14
影响因子：
4.6
通讯作者：
Perrier, Sebastien
中科院分区：
文献类型：
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作者：
Gurnani, Pratik;Floyd, Thomas;Perrier, Sebastien
合成聚合物的一些主要应用是在医疗领域（药物输送，诊断，医学成像）。然而，这些大分子的制备仅限于那些能够使用化学设施的人，这可能会延迟生物学评价。在这项工作中，我们的目标是解决这个问题，利用设备通常发现在生物实验室建立一个协议，通过RAFT聚合制备定义明确的大分子。通过利用热循环仪技术，使用丙烯酰胺单体和超快RAFT聚合，在每个加热循环的5分钟内制备均聚物、嵌段共聚物和嵌段共聚物的库。所制备的聚合物均具有良好的摩尔质量控制，并且可以在15 min内生成。
Some of the main applications for synthetic polymers are found within the medical field (drug delivery, diagnostics, medical imaging). However, preparation of these macromolecules has been limited to those with access to chemistry facilities, potentially delaying biological evaluation. In this work, we aim to address this issue by utilising equipment typically found within a biological lab to establish a protocol for the preparation of well-defined macromolecules via RAFT polymerisation. By exploiting thermocycler technology, libraries of homopolymers, block- and statistical-copolymers were prepared in under 5 minutes per heating cycle using acrylamide monomers with ultrafast RAFT polymerisation. All the polymers prepared had excellent molar mass control and could be generated in under 15 min.